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GenomicsChIP-seq of E. coli to identify H-NS and Fis binding at multiple time-points during growth in rich medium
Among the most important regulators of gene expression in bacteria are 'nucleoid-associated proteins'. These proteins alter the topology of the bound DNA by bending, wrapping or bridging it, thus having multiple effects, including transcriptional regulation, on the bacterial cell. Among the best-stu...
ORGANISM(S): Escherichia coli 
2010-09-01 | E-MTAB-332 | biostudies-arrayexpress
GenomicsSigma38 binding sites in E. Coli
ChIP-seq to identify sigma38 binding sites in wild-type and delta ssrS (6S RNA knockout) strains of E. Coli K-12 MG1655, during stationary phase ChIP-seq using antibody against sigma38 in wild-type and ssrS deletion strain. Two replicates for wild type and one replicate for ssrS deletion.
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 
2016-06-10 | E-GEOD-74836 | biostudies-arrayexpress
TranscriptomicsPsoralen binding across the Escherichia coli chromosome
We used trimethylpsoralen intercalation to map supercoiling across the E. coli chromosome. We treated E. coli cells with trimethylpsoralen and exposed them to UV light. Psoralen enters cells, intercalates between DNA base pairs, and crosslinks the two strands of DNA at a rate proportional to the loc...
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 
2016-03-22 | E-GEOD-77687 | biostudies-arrayexpress
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