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Differential expression of genes in E. coli MG1655 strains with deletions of fis and hns was assessed under early-exponential, mid-exponential, transition-to-stationary and stationary phases of growth in LB medium.
ORGANISM(S): Escherichia coli 
Among the most important regulators of gene expression in bacteria are 'nucleoid-associated proteins'. These proteins alter the topology of the bound DNA by bending, wrapping or bridging it, thus having multiple effects, including transcriptional regulation, on the bacterial cell. Among the best-stu...
ORGANISM(S): Escherichia coli 
ChIP-seq investigation of H-NS of Escherichia coli grown in the presence and absence of an antibiotic "Bicyclomycin", a potential inhibitior of transcription termination factor rho.
We used trimethylpsoralen intercalation to map supercoiling across the E. coli chromosome. We treated E. coli cells with trimethylpsoralen and exposed them to UV light. Psoralen enters cells, intercalates between DNA base pairs, and crosslinks the two strands of DNA at a rate proportional to the loc...
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 
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