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ProteomicsMethylated-antibody affinity purification to improve proteomic identification of plant RNA polymerase Pol V complex and the interacting proteins
Affinity purification followed by enzymatic digestion and mass spectrometry has been widely utilized for the sensitive detection of interacting proteins and protein complexes in various organisms. In plants, the method is technically challenging due to the low abundance proteins, non-specific bindin...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2017-02-28 | PXD005422 | Pride
On-bead digestion Pol v complex Chemical cross-link Reductive methylation Ap-ms 
ProteomicsRegion-selective and site-specific glycation of influenza proteins surrounding the viral envelope membrane
Analysis of protein modifications is critical for quality control of therapeutic biologics. We used highly sensitive LC MS/MS analyses combined with multiple enzyme digestions to determine low abundance early-stage lysine glycation products of influenza vaccines derived from embryonated chicken eggs...
ORGANISM(S): Influenza A virus (A/turkey/Italy/3466/1999(H7N1)) 
2024-10-17 | PXD052096 | Pride
Influenza vaccine Glycation Mass spectrometry 
ProteomicsTopological N-glycosylation and site-specific N-glycan sulfation of influenza proteins in the highly expressed H1N1 candidate vaccines
The outbreak of a pandemic influenza H1N1 in 2009 required the rapid generation of high-yielding vaccines against the A/California/7/2009 virus, which were achieved by either addition or deletion of a glycosylation site in the influenza proteins hemagglutinin and neuraminidase. In this report, we ha...
ORGANISM(S): Homo sapiens (Human) 
2018-11-21 | PXD007037 | Pride
Influenza h1n1 Lc ms/ms 
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