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TranscriptomicsComparison of murine colonic mucosal DNA from postnatal day 90 (P90) to postnatal day 30 (P30) by MSAM
DNA methylation profiling of colonic mucosal DNA between P90 and P30 mice. 0.5ug of DNA was serially digested with SmaI and XmaI followed by an adaptor ligation and adaptor mediated PCR amplification Two independent P90 to P30 comparisons were performed as follows. Samples were labelled with Cy3 (P3...
ORGANISM(S): Mus musculus 
2010-05-22 | E-GEOD-18031 | biostudies-arrayexpress
TranscriptomicsMethylation changes occurring in early postnatal liver development
Genomic DNA is isolated from C57BL6/J mice at E17.5 and postnatal d21, and we used MCAM to identify genes undergoing methylation change during late fetal to early postnatal mouse liver development. Two-condition experiment, P21 vs. E17.5. Biological replicates: 2 for each condition.
ORGANISM(S): Mus musculus 
2010-06-19 | E-GEOD-15634 | biostudies-arrayexpress
TranscriptomicsPostnatal epigenetic development of mouse intestinal stem cells (RNA-seq)
We used RNA sequencing to quantify the gene expression levels in the intestinal stem cells (ISCs) or their progeny during the suckling period of mouse colon development. Lgr5-EGFP mice were used to identify ISC populations in the colons. RNA sequencing was performed using EGFP labeled Lgr5+ ISCs and...
ORGANISM(S): Mus musculus 
2015-08-25 | E-GEOD-64054 | biostudies-arrayexpress
GenomicsPostnatal epigenetic development of mouse intestinal stem cells
We used unbiased whole genome bisulfite sequencing (WGBS) to identify DNA methylation changes in the intestinal stem cells (ISCs) or their progeny during the suckling period of mouse colon development. Lgr5-EGFP mice were used to identify ISC populations in the colons. WGBS were performed using EGFP...
ORGANISM(S): Mus musculus 
2015-08-25 | E-GEOD-58532 | biostudies-arrayexpress
TranscriptomicsDNA methylation and gene expression changes during human embryonic stem cell differentiation
Investigate the genome-wide DNA methylation and gene expression changes during human embryonic stem cell differentiation. hESCs (H1 and H13) DNA samples for 3 different differentian stage (D0, undifferentiated;D21, 21 days after undirected differentiation; D90, 90 days after undirected differentiati...
ORGANISM(S): Homo sapiens 
2011-09-25 | E-GEOD-32371 | biostudies-arrayexpress
TranscriptomicsInterindividual comparison of methylation profiling by MSAM
Interindividual variation in methylation profiling of human DNA samples were detected using two-tissue screening by MSAM. 0.5ug of DNA was serially digested with SmaI and XmaI followed by an adaptor ligation and adaptor mediated PCR amplification HF (hair follicle) and PBL (Peripheral Blood Leukocyt...
ORGANISM(S): Homo sapiens 
2010-12-01 | E-GEOD-19823 | biostudies-arrayexpress
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