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Parasite lysates were centrifuged (20,000 g, 4°C, 15 min). Beads (blank and with linker attached) were washed 3× with water then twice with lysis buffer. The lysate (~2 mg total protein) was first incubated with 2 mg blank beads for 30 min at 4°C with rotating agitation. The lysate was divided into ...
ORGANISM(S): Plasmodium falciparum (isolate 3D7) 
2022-10-15 | PXD033740 | Pride
Early identification of a compound’s mode of action can greatly benefit the discovery process. Thermal proteomics profiling (TPP) is a powerful, unbiased tool that can be used to identify potential ligands of protein targets. TPP takes advantage of the fact that a ligand binding to its target prot...
ORGANISM(S): Plasmodium falciparum (isolate 3D7) 
2022-10-13 | PXD025182 | Pride
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