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GenomicsChIP-Seq of ORC2 bound to third instar salivary gland DNA in WT and mutant Drosophila, analyzed by Illumina sequencing
We analyzed ORC2 ChIP-Seq from hand dissected salivary glands of wandering third instar larvae from OrR or SuUR Drosophila. Goals were to ascertain the difference in binding profile between salivary glands expressing and not expressing the Supressor of UnderReplication protein. One replicate is incl...
ORGANISM(S): Drosophila melanogaster 
2011-10-18 | E-GEOD-31899 | biostudies-arrayexpress
TranscriptomicsMurine polyploid trophoblast giant cells (TGCs) from day E9.5 implantation sites (experimental) vs E9.5 diploid embryos (control)
Array-based quantitative genome hybridization (aCGH) was performed to quantify gene copy number across the euchromatic portion of the genome of polyploid TGCs relative to embryonic diploid control cells. DNA was prepared from diploid embryonic cells and purified polyploid TGCs, labeled, and hybridiz...
ORGANISM(S): Mus musculus 
2013-04-30 | E-GEOD-45778 | biostudies-arrayexpress
TranscriptomicsEarly and late response of Nematostella vectensis transcriptome to heavy metals
Environmental contamination from heavy metals poses a global concern for the marine environment, as heavy metals are passed up the food chain and persist in the environment long after the pollution source is contained. Cnidarians, dating back to 700 million years ago, play an important role in shapi...
ORGANISM(S): Nematostella vectensis 
2015-12-22 | E-GEOD-58769 | biostudies-arrayexpress
TranscriptomicsRNA expression profiles of murine polyploid trophoblast giant cells (TGCs) from day 9.5 implantation sites.
We employed RNA-seq to transcriptionally profile a pure population of hand-dissected polyploid TGCs from embryonic day 9.5. These data provide a set of polyploid-specific TGCs transcripts that will aid in the understanding of TGCs differentiation and endoreplication. TGCs were micro-dissected from d...
ORGANISM(S): Mus musculus 
2013-04-30 | E-GEOD-45782 | biostudies-arrayexpress
TranscriptomicsmicroRNA (miRNA) expression profiles of murine polyploid trophoblast giant cells (TGCs) from day 9.5 implantation sites.
We employed miRNA-seq to profile all miRNAs from a pure population of hand-dissected polyploid TGCs from embryonic day 9.5. These data set of polyploid-specific TGCs microRNAs will provide insights into TGCs differentiation and endoreplication. TGCs were micro-dissected from day E9.5 nine implantati...
ORGANISM(S): Mus musculus 
2013-04-30 | E-GEOD-45784 | biostudies-arrayexpress
TranscriptomicsExpression profile of third instar larval salivary gland tissue
We use mRNA-seq to transcriptionally profile larval salivary gland tissue from Drosophila third instar larvae. These data provide insights into tissue physiology and can be used to identify tissue specific transcripts. Salivary glands were dissected from 200 wandering third instar larvae and the ass...
ORGANISM(S): Drosophila melanogaster 
2011-10-18 | E-GEOD-33017 | biostudies-arrayexpress
UnknownMitochondrial augmentation of CD34+ cells from healthy donors and patients with mitochondrial DNA disorders confers functional benefit.
Not available
S-EPMC8463667 | biostudies-literature
TranscriptomicsChIP with anti-orc2 antibody to identify regions of orc binding in third instar salivary glands of WT and SuUR mutant Drosophila
ChIP was performed to identify regions of gDNA bound by orc2 in third instar salivary glands of Drosophila WT and SuUR mutants. This demonstrated that ORC does not localize to regions that are under-replicated in SuUR mutant third instar salivary glands. Comparison of ORC2 ChIP sample relative to i...
ORGANISM(S): Drosophila melanogaster 
2011-10-17 | E-GEOD-31895 | biostudies-arrayexpress
TranscriptomicsChIP with anti-H3K27me3 to compare binding in salivary glands of WT and SuUR Drosophila
ChIP was performed to identify regions of gDNA bound by H3K27me3 in third instar salivary glands of Drosophila WT and SuUR mutants. This demonstrated that H3K27me3 binds differently in under-replicated in SuUR mutant third instar salivary glands. Comparison of H3K27me3 ChIP sample relative to input...
ORGANISM(S): Drosophila melanogaster 
2011-10-17 | E-GEOD-31897 | biostudies-arrayexpress
TranscriptomicsCGH to ascertain levels of gDNA in third instar salivary glands of various mutant Drosophila
Comparative genomic hybridization was performed to compare levels of gDNA in third instar salivary glands of Drosophila mutants/nulls in the SuUR and orc proteins, compared with 0-2hr diploid embryo gDNA. This illustrates regions of differential replication in the genome. CGH of salivary gland DNA ...
ORGANISM(S): Drosophila melanogaster 
2011-10-17 | E-GEOD-31898 | biostudies-arrayexpress
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