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ProteomicsTurboID-mediated proximal labeling of Drosophila BubR1 and its cleavage variants
V5-TurboID-tagged Drosophila BubR1 and its cleavage variants were transiently expressed in Drosophila S2 cells in two biological replicates. Cells were treated with 500 µM biotin for 30 min for protein biotinylation. Biotinylated protein-containing lysate was subjected to FG-NeutrAvidin beads purifi...
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
PXD033797 | JPOST Repository
ProteomicsTurboID-mediated proximal labeling of Drosophila caspase Drice in adult brains
V5-TurboID was knock-in tagged in Drosophila caspase Drice which is expressed in adult brains. Flies were fed with 100 µM biotin for protein biotinylation in two biological replicates. Biotinylated protein-containing lysate extracted from adult brains was subjected to FG-NeutrAvidin beads purificati...
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
2024-02-06 | PXD042922 | JPOST Repository
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