Sigmund Curt D CD

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Affinity purification coupled with mass spectrometry proteomics (2)
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Transcription profiling by array (2)

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Department of Physiology Medical College of Wisconsin (1)
Department of Physiology, Medical College of Wisconsin, USA (1)

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RhoBTB1 C-Terminal Binding Proteins

We identified Rho Related BTB Domain Containing 1 (RhoBTB1) as a key regulator of phosphodiesterase 5 (PDE5) activity, and through PDE5, a regulator of vascular tone. We identified the binding interface for PDE5 on RhoBTB1 by truncating full-length RhoBTB1 into its component domains. Co-immunoprecip...

ORGANISM(S): Homo sapiens (Human)

2023-08-21 | PXD043245 | Pride

RhoBTB1 C-Terminal Binding Proteins in A7r5 Smooth Muscle Cells

The RhoBTB1-Cullin3 (CUL3) pathway in smooth muscle cells (SMCs) controls the ubiquitination of target proteins and through this blood pressure (BP) and arterial stiffness. We performed APEX2 proximity labelling coupled with mass spectrometry to identify RhoBTB1 interacting partners in A7R5 SMCs. We...

ORGANISM(S): Rattus norvegicus (Rat)

2026-04-06 | PXD067797 | Pride

Proximity ligation Rhobtb1 Apex2

Gene expression profiling using exon arrays during interference with PPAR gamma signaling in thoracic aorta

Pharmacological activation of the transcription factor PPAR gamma lowers blood pressure and improves glucose tolerance in humans. In contrast, naturally occurring mutations (e.g., P467L, V290M) in the ligand binding domain of PPAR gamma in humans leads to severe insulin resistance and early-onset h...

ORGANISM(S): Mus musculus

2012-10-03 | E-GEOD-37195 | biostudies-arrayexpress

Estrogen Receptor α Is Required for Maintaining Baseline Renin Expression.

Not available

S-EPMC4833511 | biostudies-literature

CaMKII inhibition in smooth muscle cells controls Ang-II-induced gene transcription in the aorta

The Ca2+/calmodulin-dependent kinase II is expressed in smooth muscle and believed to mediate intracellular calcium handling and calcium-dependent gene transcription. CaMKII is activated by Angiotensin-II. The multifunctional calcium/calmodulin-dependent kinase II (CaMKII) is activated by Angiotensi...