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ProteomicsQuantification of mitochondrial translation by pulse SILAC
We developed a simple method that combined biochemical mitochondria isolation and pulse SILAC approach to monitor the mitochondrial translation. Our approach allows us to quantify 12 out of the 13 mitochondrial translation products, the highest coverage among analogous methods reported, and provide ...
ORGANISM(S): Homo Sapiens (human) 
2022-12-28 | PXD022476 | JPOST Repository
TranscriptomicsAnalysis of HeLa cells after transfection with miR-1, miR-124 and miR-181.
Analysis of HeLa cells at 24 hours after transfection with wild type miR-1, miR-124, miR-181 versus control transfected HeLa cells. Results were compared to protein down-regulation at 48 hours measured by SILAC-MS. Analysis of HeLa cells at 24 hours after transfection with wild type miR-1, miR-124,...
ORGANISM(S): Homo sapiens 
2010-05-25 | E-GEOD-11968 | biostudies-arrayexpress
TranscriptomicspSILAC mass spectrometry reveals ZFP91 as novel IMiD dependent substrate of the CRL4CRBN ligase
Thalidomide and its derivatives lenalidomide and pomalidomide (IMiDs) are effective treatments of hematologic malignancies. It was shown that IMiDs impart gain of function properties to the CUL4-RBX1-DDB1-CRBN (CRL4CRBN) ubiquitin ligase that enable binding, ubiquitination and degradation of key the...
ORGANISM(S): Homo sapiens 
2017-03-01 | GSE94728 | GEO
UnknownTime-resolved Analysis of Proteome Dynamics by Tandem Mass Tags and Stable Isotope Labeling in Cell Culture (TMT-SILAC) Hyperplexing.
Not available
S-EPMC5141271 | biostudies-literature
ProteomicsQuantitative proteomic analysis in Candida albicans using SILAC-based mass spectrometry
Stable isotope labelling by amino acids in cell culture (SILAC) in conjunction with mass spectrometry analysis is a sensitive and reliable technique for quantifying relative differences in protein abundance and post-translational modifications between cell populations. We have developed and utilised...
ORGANISM(S): Candida albicans (Yeast) 
2018-04-17 | PXD007825 | Pride
Nativesilac Silac Mass spectrometry Candida albicans Cdc14 
TranscriptomicsRNA-seq to investigate YBX3 siRNA knockdown in HeLa cells
This study identifies the transcriptomic effects of YBX3 depletion after 2 days of siRNA knockdown. Unspecific siRNA and untreated cells were used as controls. The cells were grown in SILAC media and the effects on protein levels were measured by mass spectrometry. Also an eCLIP (E-MTAB-5888) experi...
ORGANISM(S): Homo sapiens 
2019-05-10 | E-MTAB-7455 | biostudies-arrayexpress
ProteomicsSILAC IP-Mass spectrometry of TTLL-transfected HEK cells
Identification of the non-tubulin substrates of tubulin glycylases, TTLL3/TTLL8 and tubulin glutamylases TTLL4/TTLL5 using SILAC-IP-MS/MS quantitative mass spectrometry approach. HEK cells transfected with the respective enzymes were lysed and the proteins modified by the enzymes enriched by immunop...
ORGANISM(S): Homo sapiens (Human) 
2026-05-18 | PXD065251 | Pride
Hek cells Ttlls Ip-ms/ms Silac 
UnknownStatus of complete proteome analysis by mass spectrometry: SILAC labeled yeast as a model system.
Not available
S-EPMC1779535 | biostudies-literature
UnknownIdentification of newly translated thermo-sensitive proteins using pulse SILAC mass spectrometry and the GAL promoter system.
Not available
S-EPMC9881406 | biostudies-literature
UnknownQuantitative identification of proteins that influence miRNA biogenesis by RNA pull-down-SILAC mass spectrometry (RP-SMS).
Not available
S-EPMC6335501 | biostudies-literature
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