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TranscriptomicsTranscript profile analyses of maize silk strands during silk senescence
To fully characterize the extent of genomic changes in senescent silk, we performed a time-course tissue harvesting. Material was collected at four time points: 3-, 7-, 11- and 15 days after silk emergence (DASE). Maize inbred line B104; 2 cm of basal part of silk from rings 6-10
ORGANISM(S): Zea mays 
2022-08-16 | E-MTAB-10847 | biostudies-arrayexpress
TranscriptomicsMicroRNA expression profiling of the fifth-instar posterior silk gland of Bombyx mori
Background: The growth and development of the posterior silk gland and the biosynthesis of the silk core protein at the fifth larval instar stage of Bombyx mori are of paramount importance for silk production. Results: Here, aided by next-generation sequencing and microarry assay, we profile 1,229 m...
ORGANISM(S): Bombyx mori 
2014-07-30 | E-GEOD-56380 | biostudies-arrayexpress
ProteomicsWestern black widow major ampullate silk gland and silk fiber LC-MSMS, Part 1
Spider silk research has largely focused on spidroins, proteins that are the primary components of spider silk fibers. Although a number of spidroins have been characterized, other types of proteins associated with silk synthesis are virtually unknown. Previous comparison of tissue-specific RNAseq l...
ORGANISM(S): Latrodectus hesperus (Western black widow spider) 
2015-08-25 | PXD002592 | Pride
Major ampullate silk fibers Spidroins Spider Latrodectus hesperus Draglines Major ampullate silk glands 
TranscriptomicsIdentification of KIL1 targets via RNA-Seq in Zea mays
To understand how the NAC transcription factor KIL1 regulates age-induced senescence and cell death in maize silks, we need to get a genome-wide view on its downstream targets. We propose to compare the transcriptome profiles of GOF and LOF transgenic silk tissue with the profile of wild-type B104 s...
ORGANISM(S): Zea mays 
2022-08-16 | E-MTAB-10850 | biostudies-arrayexpress
ProteomicsProteome identification of the silkworm middle silk gland
The middle silk gland (MSG) is an important silkworm sub-organ for synthesis and secretion of sericin proteins. To investigate the proteome differences among the anterior, middle, and posterior regions of silkworm MSG at the third day of the fifth instar, the extracted tissue proteins were digested ...
ORGANISM(S): Bombyx mori (Silk moth) 
2016-03-11 | PXD003371 | Pride
Shotgun lc-ms/ms Proteomics Middle silk gland Silkworm 
ProteomicsWestern black widow major ampullate, minor ampullate, and tubuliform silk gland LC-MSMS, Part 2
Spider silk research has largely focused on spidroins, proteins that are the primary components of spider silk fibers. Although a number of spidroins have been characterized, other types of proteins associated with silk synthesis are virtually unknown. Previous comparison of tissue-specific RNAseq l...
ORGANISM(S): Latrodectus hesperus (Western black widow spider) 
2015-08-25 | PXD002595 | Pride
Minor ampullate silk glands Spidroins Spider Tubuliform silk glands Latrodectus hesperus Major ampullate silk glands 
TranscriptomicsExpression data from silkworm posterior silk glands at the late larval stages
The silkworm silk gland is one of the most efficient protein synthesis systems among all organisms. Its amazingly efficient protein synthesis makes the silk gland a desirable object for basic studies on gene expression and regulation and for biotechnological applications. At the early stages of the ...
ORGANISM(S): Bombyx mori 
2012-12-05 | E-GEOD-26172 | biostudies-arrayexpress
TranscriptomicsBombyx mori Posterior and Median Silk Gland SAGE transcriptome
To identify functions that distinguish the posterior and median cells producing fibroin and sericin in the silk gland of Bombyx mori, serial analysis of gene expression (SAGE) profiles from both silk gland regions were analyzed and compared. The construction of a B. mori reference tag collection ext...
ORGANISM(S): Bombyx mori 
2010-12-22 | E-GEOD-23098 | biostudies-arrayexpress
ProteomicsLC-MS/MS data from spider major ampullate silk fibers and glands
This project contains proteomic (LC-MS/MS) data from 27 samples. The samples are as follows: (1) Major ampullate glands dissolved in 8M Urea (3 replicates); (2) Major ampullate silk fibers dissolved in 8M Urea (3 replicates); (3) Major ampullate silk fibers dissolved in Hexafluoroisopropanol (3 re...
ORGANISM(S): Larinioides sclopetarius 
2024-07-16 | PXD052116 | Pride
Major ampullate silk Major ampullate glands Spider silk Lc-ms/ms 
TranscriptomicsMicroRNAs of Bombyx mori identified by Solexa sequencing
Background: MicroRNA (miRNA) and other small regulatory RNAs contribute to the modulation of a large number of cellular processes. We sequenced three total RNA libraries prepared from the whole body, and the anterior and posterior silk glands of Bombyx mori, with a view to expanding the repertoire o...
ORGANISM(S): Bombyx mori 
2010-02-01 | E-GEOD-17965 | biostudies-arrayexpress
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