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MetabolomicsTargeting methionine synthase in a fungal pathogen causes a metabolic imbalance that impacts cell energetics, growth and virulence
There is an urgent need to develop novel antifungals to tackle the threat that fungal pathogens pose to human health. In this work, we have performed a thorough characterisation and validation of the promising target methionine synthase. We have uncovered that in Aspergillus fumigatus the absence of...
2020-10-15 | MTBLS1636 | MetaboLights
MetabolomicsMetabolomic analysis of riboswitch containing E. coli recombinant expression system
In this study we have employed metabolomics approaches to understand the metabolic effects of producing enhanced green fluorescent protein (eGFP) as a recombinant protein in Escherichia coli cells. This metabolic burden analysis was performed against a number of recombinant expression systems and co...
2017-11-16 | MTBLS393 | MetaboLights
MetabolomicsMetabolomics Analysis Reveals Tissue-Specific Metabolite Compositions in Leaf Blade and Traps of Carnivorous Nepenthes Plants
Nepenthes is a genus of carnivorous plants that evolved a pitfall trap, the pitcher, to catch and digest insect prey to obtain additional nutrients. Each pitcher is part of the whole leaf, together with a leaf blade. These two completely different parts of the same organ were studied separately in a...
2020-07-10 | MTBLS1783 | MetaboLights
TranscriptomicsRNA-seq analysis of vorinostat-resistant HCT116 cells following gene knockdown of GLI1 or PSMD13 with or without vorinostat treatment
Transcriptome analysis was conducted on vorinostat resistant HCT116 cells (HCT116-VR) upon knockdown of potential vorinostat resistance candidate genes in the presence and absence of vorinostat. Potential vorinostat resistance candidate genes chosen for this study were GLI1 and PSMD13, which were i...
ORGANISM(S): Homo sapiens 
2016-06-30 | E-GEOD-57871 | biostudies-arrayexpress
TranscriptomicsExpression data from SGBS human cells before and after 24 hours of stimulation with differentiation cocktail, with or without Scrambled (Scr) or Tenomodulin (TNMD) siRNA to knockdown the genes of interest.
In a screen for upregulated adipocyte genes in insulin resistant versus insulin sensitive subjects matched for BMI, we identified the type II transmembrane protein tenomodulin (TNMD), previously implicated in glucose tolerance in gene association studies. TNMD expression was greatly increased in hum...
ORGANISM(S): Homo sapiens 
2016-02-11 | E-GEOD-76319 | biostudies-arrayexpress
TranscriptomicsIntracellular and extracellular microRNAs expressed by HEK293T cells
MicroRNAs (miRNAs) are a class of small RNA molecules that regulate expression of specific mRNA targets. They can be released from cells, often encapsulated within extracellular vesicles (EVs), and therefore have the potential to mediate intercellular communication. It has been suggested that certai...
ORGANISM(S): Homo sapiens 
2012-06-25 | E-GEOD-38916 | biostudies-arrayexpress
UnknownLost microbes of COVID-19: <i>Bifidobacterium</i>, <i>Faecalibacterium</i> depletion and decreased microbiome diversity associated with SARS-CoV-2 infection severity.
Not available
S-EPMC9051551 | biostudies-literature
TranscriptomicsA sputum gene expression signature of six biomarkers identifies asthma inflammatory phenotype and steroid responsiveness
This study identifies differentially expression genes in the sputum of people with eosinophilic, neutrophilic and paucigranulocytic asthma. A selection of markers identified using this microarray were further validated using qPCR on a wider sample set. Gene expression profiles were generated from in...
ORGANISM(S): Homo sapiens 
2014-03-25 | E-GEOD-45111 | biostudies-arrayexpress
UnknownInterventions for prevention of childhood obesity in primary care: a qualitative study.
Not available
S-EPMC4933605 | biostudies-literature
TranscriptomicsNon-coding small RNA profiling by high throughput sequencing of bovine primary retinal microvascular endothelial cells
The aim of this study was to identify and quantify microRNAs and other small regulatory RNAs expressed in primary retinal microvascular endothelial cells (RMECs) using deep sequencing. RMECs were isolated, RNA extracted, a small RNA library prepared and deep sequencing performed. A total of 6.8 mill...
ORGANISM(S): Bos taurus 
2012-02-13 | E-GEOD-31340 | biostudies-arrayexpress
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