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Mice underwent cecal ligation and puncture (19 GA with concurrent administration of imipenem), sham operation, or were unoperated. 14 days after operation, microglia were isolated by fluorescent activated cell sorting for CD11b+/CD45mid/CD64+ cells. For each sample, the brains of 3 mice were poole...
ORGANISM(S): Mus musculus 
We report gene expression changes in Cul3 deficient thymic CD4+ T cells We used microarrays to detail the global programme of gene expression changes upon removal of Cul3 during thymic development CD25-, CD1dPBS57-, gdTCR - CD4+ Single positive cells from thymocytes of CD4Cre-Cul3fl/fl (LM) and CD4 ...
ORGANISM(S): Mus musculus 

Pulmonary fibrosis is a heterogenous syndrome in which fibrotic scar replaces normal lung tissue. We performed massively parallel single-cell RNA-Seq on lung tissue from eight lung transplant donors and eight patients with pulmonary fibrosis. Combined with in situ RNA hybridization, with amplific...

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