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TranscriptomicsCloning of 50-75 nt small RNAs associated with Armitage in OSCs
Armitage (Armi) is a component of Yb bodies and functions in the primary piRNA processing pathway. Armi interaction with Piwi does not require Yb expression; however, without Yb, the Armi-Piwi complex does not localize at Yb bodies and contains few piR-ILs (small RNAs associated with Armitage compl...
ORGANISM(S): Drosophila melanogaster 
2010-10-24 | E-GEOD-23931 | biostudies-arrayexpress
TranscriptomicsHITS-CLIP analysis of Yb binding sites in Drosophila ovary cell line
piRNAs direct Piwi to repress transposons to maintain genome integrity in Drosophila ovarian somatic cells. piRNA maturation and association with Piwi occur at perinuclear Yb bodies, the centers of piRNA biogenesis. Here, we show that piRNA intermediates arising from the piRNA cluster flamenco (flam...
ORGANISM(S): Drosophila melanogaster 
2014-06-24 | E-GEOD-54875 | biostudies-arrayexpress
UnknownCpf1 nucleases demonstrate robust activity to induce DNA modification by exploiting homology directed repair pathways in mammalian cells.
Not available
S-EPMC5024423 | biostudies-literature
TranscriptomicsFLX sequencing of Piwi-associated small RNAs extracted from Drosophila ovarian somatic cells (OSC)
piRNAs function in silencing retrotransposons by associating with the PIWI proteins, AGO3, Aub, and Piwi, in Drosophila germlines. Bioinformatics analyses of piRNAs in Drosophila ovaries suggested that piRNAs are produced by two systems, the primary processing pathway and the amplification loop, fro...
ORGANISM(S): Drosophila melanogaster 
2009-10-01 | E-GEOD-15137 | biostudies-arrayexpress
UnknownZFAS1: a long noncoding RNA associated with ribosomes in breast cancer cells.
Not available
S-EPMC5117590 | biostudies-literature
TranscriptomicsSomatic Primary piRNA Biogenesis Driven by Cis-Acting RNA Elements and Trans- Acting Yb
Primary piRNAs in Drosophila ovarian somatic cells arise from piRNA cluster transcripts and the 3′ UTRs of a subset of mRNAs, including Traffic jam (Tj) mRNA. However, it is unclear how these RNAs are determined as primary piRNA sources. Here, we identify a cis-acting 100-nt fragment in the Tj 3′ UT...
ORGANISM(S): Drosophila melanogaster 
2015-06-05 | E-GEOD-69625 | biostudies-arrayexpress
ProteomicsMass spectrometry for SUMO site detection from 3×Flag-SUMO-T86K expressing cells
Anti-Flag immunoprecipitated 3×Flag-SUMO-T86K proteins on beads were washed once and suspended in 50 mM ammonium bicarbonate, followed by the addition of 1 µg Lys-C and an overnight incubation at 37ºC. The digests were reduced, alkylated, diluted 10-fold with HBS (50 mM HEPES-NaOH pH7.5, 150 mM NaCl...
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
2025-09-19 | PXD068512 | JPOST Repository
ProteomicsMass spectrometry for SUMO site detection from 3×Flag-SUMO-T86R expressing cells
Anti-Flag immunoprecipitated 3×Flag-SUMO-T86R proteins on beads were washed once and suspended in 50 mM ammonium bicarbonate, followed by the addition of 1 µg trypsin/Lys-C mix and an overnight incubation at 37ºC. The digests were reduced, alkylated, diluted 10-fold with HBS (50 mM HEPES-NaOH pH7.5,...
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
2025-09-19 | PXD068511 | JPOST Repository
ProteomicsProteomic analysis of PNUTS-associated proteins immunopurified with an anti-HA antibody
Immunoprecipitated proteins on beads were washed once and resuspended in 50 mM ammonium bicarbonate, followed by the addition of 200 ng trypsin/Lys-C mix and an overnight incubation at 37ºC. The digests were reduced, alkylated, acidified, and desalted with GL-Tip SDB. Then, the eluates were evaporat...
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
2025-09-19 | PXD068513 | JPOST Repository
ProteomicsProteomic analysis of SovN-associated proteins immunopurified with an anti-FLAG antibody
Immunoprecipitated proteins on beads were washed once and resuspended in 50 mM ammonium bicarbonate, followed by the addition of 200 ng trypsin/Lys-C mix and an overnight incubation at 37ºC. The digests were reduced, alkylated, acidified, and desalted with GL-Tip SDB. Then, the eluates were evaporat...
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
2025-09-19 | PXD068509 | JPOST Repository
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