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TranscriptomicsChip-chip from E. coli MG1655 cells with different methods to detect SeqA and σ32 binding and causes of high background
Reinvestigation of the chromatin immunoprecipitation procedure led us to discover four causes of high background: non-unique sequences, incomplete reversion of crosslinks, washing with spin-columns and insufficient RNase treatment. We used a publishd method giving a high background signal and a modi...
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 
2010-07-12 | E-GEOD-19053 | biostudies-arrayexpress
TranscriptomicsSeqA Chip-chip analysis of E. coli MG1655 and derivatives
Chromosomes are composed of enormously long DNA molecules which must be distributed correctly as the cells grow and divide. In Escherichia coli the new DNA behind the replication forks is specifically bound by the SeqA protein. SeqA binds to GATC sequences which are methylated on the A of the old st...
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 
2012-02-22 | E-GEOD-28280 | biostudies-arrayexpress
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