SeqA Chip-chip analysis of E. coli MG1655 and derivatives

ABSTRACT: Chromosomes are composed of enormously long DNA molecules which must be distributed correctly as the cells grow and divide. In Escherichia coli the new DNA behind the replication forks is specifically bound by the SeqA protein. SeqA binds to GATC sequences which are methylated on the A of the old strand but not on the new strand. Binding lasts for a period of time until Dam methyltransferase methylates the new strand. It is therefore believed that a region of hemi-methylated DNA covered by SeqA follows the replication fork. We show that this is indeed the case by using global ChIP on Chip analysis and a newly developed method for methylation analysis. A comparison of rapid and slow growth conditions showed that in cells with multiple replication forks per chromosome, the old forks bind lit

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655

SUBMITTER: Torsten Waldminghaus 

PROVIDER: E-GEOD-28280 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress