Both SKOV3 and IOSE80 cells were cultured and extracted the proteins. With sequential digestion by trypsin, enriched by Zwitterionic Hydrophilic Interaction chromatography (ZIC-HILIC), the intact N-glycopeptides were then isotopic diethyl labelled and analyzed using C18-RPLC-MS/MS (HCD).
To determine the lncRNA and mRNA expression profile in SKOV3/SKOV3_DDP/IOSE80, we uesed lncRNA microArray analysis form Arraystar to examine the expression of lncRNAs and mRNAs in SKOV3/SKOV3_DDP/IOSE80.
To identify the downstream mRNA targeted by CDIPTOSP/STAU1 for degradation, RNA-Seq analyses were performed on SKOV3 cells transfected with si-NC (control), si-CDIPTOSP, and si-STAU1.
Background: Epithelial ovarian carcinoma (EOC) is a malignant tumor with high motility in women. Our previous study found that dysregulated NTPCR was associated with the prognosis of ovarian cancer patients, and thus this present study attempted to explore the potential roles of nucleoside-triphosph...
