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Comparative glycomics study on the surface of SKOV3 versus IOSE80 cell lines

ABSTRACT: Both SKOV3 and IOSE80 cells were cultured and extracted the proteins. With sequential digestion by trypsin, enriched by Zwitterionic Hydrophilic Interaction chromatography (ZIC-HILIC), the intact N-glycopeptides were then isotopic diethyl labelled and analyzed using C18-RPLC-MS/MS (HCD).

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell

DISEASE(S): Malignant Neoplasm Of Ovary

SUBMITTER: Ying Zhou

LAB HEAD: Ying Zhou

PROVIDER: PXD030927 | Pride | 2023-03-10

REPOSITORIES: Pride

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Comparative glycoproteomics study on the surface of SKOV3 versus IOSE80 cell lines.

Zhou Ying Y Cai Xiaoyu X Wu Linwen L Lin Nengming N

Frontiers in chemistry 20221122

Objective: Site- and structure-specific quantitative N-glycoproteomics study of differential cell-surface N-glycosylation of ovarian cancer SKOV3 cells with the non-cancerous ovarian epithelial IOSE80 cells as the control. Methods: C18-RPLC-MS/MS (HCD with stepped normalized collision energies) was used to analyze the 1: 1 mixture of labeled intact N-glycopeptides from SKOV3 and IOSE80 cells, and the site- and structure-specific intact N-glycopeptide search engine GPSeeker w ...[more]

PMID: 36482940

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