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TranscriptomicsNext generation sequencing of small RNAs isolated from exosomes in human semen
Preparation of exosomes isolated from semen contain a substantial amount of RNA, mostly from 20 to 100 nucleotides in length. We sequenced separately 20-40 and 40-100 nucleotide fractions of RNA from exosomes isolated from semenal fluid from six healthy donors. We found various classes of small non...
ORGANISM(S): Homo sapiens 
2014-06-04 | E-GEOD-56076 | biostudies-arrayexpress
TranscriptomicsRNA at 92 M-BM-0C: the non-coding transcriptome of the hyperthermophilic archaeon Pyrococcus abyssi.
The non-coding transcriptome of the hyperthermophilic archaeon Pyrococcus abyssi is investigated using the RNA-seq technology. A dedicated computational pipeline analyzes RNA-seq reads and prior genome annotation to identify small RNAs, untranslated regions of mRNAs, and cis-encoded antisense transc...
ORGANISM(S): Pyrococcus abyssi 
2014-05-31 | E-GEOD-58131 | biostudies-arrayexpress
TranscriptomicsRNA sequencing identifies specific PIWI-interacting small non-coding RNA expression patterns in breast cancer
PIWI-interacting RNAs (piRNAs) are a novel class of small ncRNAs initially isolated from germ line cells; although recent studies report that they are expressed also in somatic cells. To elucidate the role of piRNAs in somatic cells, in particular from breast cancer, we performed the first extensive...
ORGANISM(S): Homo sapiens 
2014-10-15 | E-GEOD-56134 | biostudies-arrayexpress
TranscriptomicsNon-coding RNA tiling array in rice
The 1 Mb tiling array was used to represent the 10.0 Mb to 11.0 Mb region of japonica rice Chromosome 10. A tile path was designed with 36-mer probes at a step of 5 nt. All 388,994 probes were retained and synthesized into a single array. This array was hybridized to the cDNA target used for the ful...
ORGANISM(S): Oryza sativa 
2010-06-11 | E-GEOD-6922 | biostudies-arrayexpress
TranscriptomicsChanges in expression of small non-coding RNAs/microRNAs during aging in C. elegans
We report a significant number of miRNAs and other non-coding small RNAs show dramatic changes in expression during aging in C. elegans. RNAs were prepared from four diffrent timepoints during adulthood of C. elegans hermaphrodites, Day 0, 5, 8 and 12 post-L4 molt, and used for making cDNA libraries...
ORGANISM(S): Caenorhabditis elegans 
2009-12-31 | E-GEOD-18634 | biostudies-arrayexpress
ProteomicsComprehensive Identification of Soybean Long Non-coding RNAs Reveals a Subset of Small Peptide-Coding Transcripts
Long non-coding RNAs (lncRNAs) are defined as non-protein-coding transcripts that are at least 200 nucleotides long. They are known to play pivotal roles in regulating gene expression, especially during stress responses in plants. We used a large collection of in-house transcriptome data from variou...
ORGANISM(S): Glycine max 
2020-01-09 | PXD014553 | Pride
Long non-coding rna Proteomics Soybean Small peptide Transcriptomics 
TranscriptomicsGenome-wide profiling of miRNAs and other small non-coding RNAs in the Verticillium dahliae inoculated cotton roots
MicroRNAs (miRNAs) and small interfering RNAs (siRNAs) are short (19–25 nucleotides) non-coding RNA molecules that have large-scale regulatory effects on development and on stress responses in plants.The objective of this study is to investigate the transcriptional profile of miRNAs and other smal...
ORGANISM(S): Gossypium hirsutum 
2011-04-29 | E-GEOD-28236 | biostudies-arrayexpress
TranscriptomicsSmall non-coding RNA profiling and the role of piRNA pathway genes in the protection of chicken primordial germ cells
Background: Genes, RNAs, and proteins play important roles during germline development. However, the functions of non-coding RNAs (ncRNAs) on germline development remain unclear in avian species. Recent high-throughput techniques have identified several classes of ncRNAs, including micro RNAs (miRNA...
ORGANISM(S): Gallus gallus 
2015-04-27 | E-GEOD-60400 | biostudies-arrayexpress
TranscriptomicsProfile of small RNA transcriptome during cortical development in the Rat
Small non-coding RNAs play important roles in regulating gene expression during development and disease. However, a comprehensive analysis of small RNAs in developing brain is largely lacking. By using next-generation sequencing technique, we carried out a systematic analysis of small RNA transcript...
ORGANISM(S): Rattus norvegicus 
2012-06-25 | E-GEOD-27576 | biostudies-arrayexpress
TranscriptomicsThe non-coding RNA of the multidrug resistance-linked vault particle encodes multiple regulatory small RNAs
Vault particles are conserved organelles that have been implicated in multidrug resistance and intracellular transport. They are formed by three different proteins and the non-coding vault RNAs (vRNAs). Here we show that human vRNAs produce several small RNAs (svRNAs) by mechanisms different from th...
ORGANISM(S): Homo sapiens 
2009-09-07 | E-GEOD-16060 | biostudies-arrayexpress
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