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CD4 T cells taken from 6 donors were sorted into CD25hi CD45RA+ (na�ve Treg), CD25hi CD45RO+ (memory Treg), CD25- CD45RA+ (na�ve responder) and CD25- CD45RO+ (memory responder) populations.

RNA was extracted (Ambion RNAqueous), amplified (SMART), labelled with cy3, mixed with cy5 labelle...
ORGANISM(S): Homo sapiens 
In multiple myeloma, the objective is to assess the impact of syntenin knockout on the secretory profile of bone marrow stromal cells using the HS-5 model.
ORGANISM(S): Homo sapiens (Human) 
2025-07-26 | PXD064458 | Pride
Fibroblasts, Serum-free Fibrocytes, Serum-containing Fibrocytes, Monocytes, Macrophages, Osteoclasts, immature Dendritic Cells, and mature Dendritic Cells were all generated from 3 biological replicates from each of 3 separate donors. RNA was extracted (Ambion RNAqueous), labelled with cy3, mixed w...
ORGANISM(S): Homo sapiens 

Root mutualistic microbes can modulate the production of plant secondary metabolites affecting plant–herbivore interactions. Still, the main mechanisms underlying the impact of root mutualists on herbivore performance remain ambiguous. In particular, little is known about how changes in the plant...

2023-09-12 | MTBLS2700 | MetaboLights
PBMCs from 4 donors were stained with the influenza A virus HLA-A24/PB1 498-505 tetramer. Two donors (non-LIFT8 and non-LIFT12) showed TCR independent KIR binding to tetramers whereas the other two donors (Non-LIFT14 and Non-LIFT10) showed TCR specific binding. Cells were single-cell sorted on a BD ...
ORGANISM(S): Homo sapiens 
Hybrid (Bound) and non-hybrid (Unbound) DNA from Hela EV was isolated using DRIP procedure as follows. Between 1-5μg of nucleic acid DNA from EVs (DNase0 treated) was incubated overnight with 1μg of anti-DNA-RNA Hybrid (S9.6) antibody (KeraFast) under rotation at 4°C in 500μl of binding buffer (10mM...
ORGANISM(S): Homo sapiens 
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