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TranscriptomicsSMAUG is required for zygotic transcription during the maternal-zygotic transition MZT
Microarray analysis of zygotic gene expression in 2-to-3 hour wild-type (wt) and smg mutant embryos. Expression is relative to mature, stage 14 oocytes, which contain the full maternal pool of mRNA. Strictly maternal genes that are not transcribed at the MZT contribute approximately 80% of transc...
ORGANISM(S): Drosophila melanogaster 
2010-06-19 | E-GEOD-13287 | biostudies-arrayexpress
TranscriptomicsGlobal regulation of mRNA translation and stability in the early Drosophila embryo by the Smaug RNA-binding protein- Polysome gradient/microarray study
To gain a panoramic view of Smaug function in the early embryo we identified mRNAs that are bound to Smaug using RNA co-immunoprecipitation followed by hybridization to DNA microarrays. We also identified the mRNAs that are translationally repressed by Smaug using polysome gradients and microarrays....
ORGANISM(S): Drosophila melanogaster 
2013-12-03 | E-GEOD-50026 | biostudies-arrayexpress
TranscriptomicsSMAUG Is a Major Regulator of Maternal mRNA Destabilization in Drosophila
In animals, egg activation triggers a cascade of posttranscriptional events that act on maternally synthesized RNAs. We show that, in Drosophila, the PAN GU (PNG) kinase sits near the top of this cascade, triggering translation of SMAUG (SMG), a multifunctional posttranscriptional regulator conserve...
ORGANISM(S): Drosophila melanogaster 
2007-09-13 | E-GEOD-8910 | biostudies-arrayexpress
TranscriptomicsmiR-309-dependent unstable maternal mRNAs are stabilized in smaug mutants
The behavior of 410 miR-309-dependent maternal mRNAs (from Bushati et al., 2008) in embryos from wild type and smg-mutant females relative to wild-type stage 14 oocyte reference RNA. All are unstable in wild-type while almost 85% are stabilized in smg mutants mRNA was extracted from staged embryos a...
ORGANISM(S): Drosophila melanogaster 
2010-06-19 | E-GEOD-13288 | biostudies-arrayexpress
TranscriptomicsIdentification of Smaug target mRNAs in the early Drosophila embryo using RIP-Chip
To identify SmaugM-bM-^@M-^Ys target mRNAs on a genome-wide scale we used ribonucleoprotein (RNP) co-immunoprecipitation followed by microarray analysis of the co-purifying mRNAs (RIP-Chip). Extracts, prepared from wild-type embryos collected 0-3 hours post-egglaying, were immunoprecipitated with an...
ORGANISM(S): Drosophila melanogaster 
2013-12-03 | E-GEOD-49943 | biostudies-arrayexpress
TranscriptomicsExpression analysis of Drosophila melanogaster's primordial germ cells and somatic cells in wild type and smaug mutants
Background: During the maternal-to-zygotic transition (MZT) vast changes in the embryonic transcriptome are produced by a combination of two processes: elimination of maternally provided mRNAs and synthesis of new transcripts from the zygotic genome. Previous genome-wide analyses of the MZT have bee...
ORGANISM(S): Drosophila melanogaster 
2012-02-08 | E-GEOD-34397 | biostudies-arrayexpress
UnknownSMAUG: Analyzing single-molecule tracks with nonparametric Bayesian statistics.
Not available
S-EPMC7529709 | biostudies-literature
TranscriptomicsThe Smaug RNA-binding protein is essential for microRNA synthesis during the Drosophila maternal-to-zygotic transition
Background: Metazoan embryos undergo a maternal-to-zygotic transition (MZT) during which a subset of maternal gene products is eliminated and the zygotic genome becomes transcriptionally active. RNA-binding proteins (RBPs) and the microRNA-induced silencing complex (miRISC) – of which Argonaute 1 ...
ORGANISM(S): Drosophila melanogaster 
2016-09-06 | E-GEOD-82194 | biostudies-arrayexpress
UnknownATP is dispensable for both miRNA- and Smaug-mediated deadenylation reactions.
Not available
S-EPMC5435859 | biostudies-literature
TranscriptomicsGlobal regulation of mRNA translation and stability in the early Drosophila embryo by the Smaug RNA-binding protein- Polysome gradient/microarray study
To gain a panoramic view of Smaug function in the early embryo we identified mRNAs that are bound to Smaug using RNA co-immunoprecipitation followed by hybridization to DNA microarrays. We also identified the mRNAs that are translationally repressed by Smaug using polysome gradients and microarrays....
ORGANISM(S): Drosophila melanogaster 
2013-12-03 | GSE50026 | GEO
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