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TranscriptomicsArray results for differential gene expression in 661W, a mouse cone photoreceptor-derived cell line, comparing cells incubated with vehicle controls vs. those treated with either of two 7-dehdyrocholesterol-derived oxysterols or cholesterol
RELEVANCE: Smith-Lemli-Opitz syndrome (SLOS) is a human disease caused by mutations in the gene coding for the enzyme DHCR7 (7-dehydrocholesterol (7DHC) reductase), which catalyzes the final step of cholesterol biosynthesis. Accumulated 7DHC in tissues and body fluids of SLOS patients gives rise t...
ORGANISM(S): Mus musculus 
2021-03-20 | E-MTAB-10055 | biostudies-arrayexpress
TranscriptomicsAlterations in soybean gene expression profile after foliar application of lipo-chitooligosaccharide (LCO) from Bradyrhizobium japonicum under sub-optimal temperature
Lipo-chitooligosaccharides (LCOs) produced by N2-fixing rhizobacteria initiate host nodule formation. Foliar application of LCOs has been shown to induce stress-related genes under optimal growth conditions. To study the effects of LCO foliar spray under stressed conditions, soybean seedlings grow...
ORGANISM(S): Glycine max 
2010-03-20 | E-GEOD-20972 | biostudies-arrayexpress
TranscriptomicsTranscriptional changes in soybean nodules with the loss of expression of GmbHLHm1
This study was conducted to investigate the changes in gene expression that occurs with the silencing of GmbHLHm1. GmbHLHm1 is a membrane localised DNA binding transcription factor that is important for nodule development. Its activity is linked to ammonium transport. 7 samples
ORGANISM(S): Glycine max 
2014-03-14 | E-GEOD-55896 | biostudies-arrayexpress
TranscriptomicsSuhB associates with Nus factors to facilitate 30S ribosome biogenesis in Escherichia coli
This experiment consists of two studies. The first where two independent biological replicates of MG1655, MG1655 thyA suhB::thyA and MG1655 thyA nusB::thyA were each grown in LB to mid-exponential phase. RNA-seq and associated data analysis were performed as described previously (Stringer et al., 20...
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 
2016-01-20 | E-MTAB-4240 | biostudies-arrayexpress
TranscriptomicsThe Fusarium graminearum Histone H3 K27 Methyltransferase KMT6 Regulates Development and Expression of Secondary Metabolite Gene Clusters
We performed ChIP-seq of histone modifications and RNA-seq in WT and kmt6 mutant Fusarium graminearum grown in high and low nitrogen conditions. Two replicates of RNA seq for WT and kmt6 strains at high and low nitrogen. Four histone modification ChIPs in WT and kmt6 mutant strains at high and low n...
ORGANISM(S): Fusarium graminearum 
2014-02-27 | E-GEOD-50689 | biostudies-arrayexpress
TranscriptomicsGenome-Scale Mapping of Escherichia coli σ54 Reveals Widespread, Conserved Intragenic Binding
We investigated the effect that intragenic s54 binding sites have on transcripton elongation. In an rpoN deletion we have over-expressed rpoN from a plasmid (with vector only contol) and then looked at changes in transcription elongation around intragenic s54 binding sites by RNA-seq. We are also lo...
ORGANISM(S): Escherichia coli 
2015-08-06 | E-MTAB-3699 | biostudies-arrayexpress
TranscriptomicsRNA-seq of MG1655 wt and MG1655 delta-phoB strains grown in MOPS media with low phosphate (0.2 mM)
We used RNA-seq to determine differences in RNA levels between wild-type E. coli K-12 and a ΔphoB mutant, each grown in MOPS media with low phosphate (0.2 mM).
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 
2020-07-12 | E-MTAB-9292 | biostudies-arrayexpress
TranscriptomicsRNA-seq of MG1655 wt and MG1655 delta-phoB strains grown in MOPS media with low phosphate (0.2 mM)
We used RNA-seq to determine differences in RNA levels between wild-type E. coli K-12 and a ΔphoB mutant, each grown in MOPS media with low phosphate (0.2 mM).
ORGANISM(S): Escherichia coli O17 
2020-10-03 | E-MTAB-9591 | biostudies-arrayexpress
UnknownSoybean SAT1 (Symbiotic Ammonium Transporter 1) encodes a bHLH transcription factor involved in nodule growth and NH4+ transport.
Not available
S-EPMC3977234 | biostudies-literature
TranscriptomicsMapping the Regulatory Network for Salmonella Typhimurium Invasion
Using RNAseq, the goal of the study is to investigate transcription factor regulation of virulence genes in 14028s Salmonella Typhimurium grown in SPI-1-inducing conditions. Transcription factors were cloned into pBAD24. RNA levels were compared in 14028s Salmonella Typhimurium with the transcriptio...
ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium 
2015-08-17 | E-MTAB-3765 | biostudies-arrayexpress
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