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MetabolomicsPROMIS, global analysis of PROtein-Metabolite Interactions using Size separation in Arabidopsis thaliana
Small molecules not only represent cellular building blocks and metabolic intermediates, but also regulatory ligands and signaling molecules that interact with proteins. Although these interactions affect cellular metabolism, growth, and development, they have been largely understudied. Herein, we d...
2018-07-19 | MTBLS94 | MetaboLights
ProteomicsTusA influences Fe-S cluster assembly and iron homeostasis in E. coli by reducing the translation efficiency of Fur
To investigate the influence of iron and the ΔtusA and ΔmnmA deletion mutations on the overall protein abundance, a detailed proteomic analysis was performed. Cells of both strains and the corresponding BW25113 parental strain were cultivated in medium with KNO3 as electron acceptor and iron was rem...
ORGANISM(S): Escherichia coli 
2024-05-20 | PXD052252 | Pride
E.coli Lcmsms 
ProteomicsDynamic maps of protein-protein-metabolite complexes of Arabidopsis Thaliana seedlings
The project aimed to create dynamic maps of protein-protein-metabolite complexes of Arabidopsis thaliana seedlings using PROMIS (PROtein–Metabolite Interactions using Size separation). The approach involves using size exclusion chromatography (SEC) to separate complexes, followed by LC-MS-based pro...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-10-14 | PXD026713 | Pride
Protein-metabolite complexes Ligand Protein-protein complexes 
ProteomicsInteraction partners of the K(+) efflux antiporter 3
Thylakoids were isolated, solubilized and separated by BN-PAGE in order to determinate complex compositions in different genotypes
ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress) 
2021-04-01 | PXD019865 | JPOST Repository
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