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We developed a hanging drop (HD) method to improve the sample recovery for spatial proteomics by placing the nanowell chip in an upside-down direction during droplet-based sample preparation. Compared with the commonly-used sitting-drop (SD) method, the HD method can keep the tissue voxel away from ...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2022-04-21 | MSV000089295 | MassIVE
Single cell proteomics data from three murine cell lines (Raw, SVEC4-10, C10) and HeLa cell lysate. Samples were digested with trypsin and labeled with TMT 10-Plex on a small scale using nanoPOTS, followed by LC-MS/MS analysis.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Mus Musculus (ncbitaxon:10090) 
2019-07-19 | MSV000084110 | MassIVE
Here we report a facile method based on in situ chemical derivatization followed by extraction for analysis of metabolites and other chemicals in hypersaline samples, enabling for the first time direct LC-MS-based exo-metabolomics analysis in sample matrices containing up to 2 molar total dissolved ...
ORGANISM(S): Environmental Samples <verrucomicrobiales> (ncbitaxon:48468) 
2020-07-10 | MSV000085713 | GNPS
We report a facile one-pot nanoproteomics method termed SOPs-MS (Surfactant-assisted One-Pot sample processing at the standard volume coupled with MS) for convenient proteome profiling of 50-1000 mammalian cells. Building upon our recent development of SOP-MS for label-free single-cell proteomics at...
ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse) 
We report a facile one-pot nanoproteomics method termed SOPs-MS (Surfactant-assisted One-Pot sample processing at the standard volume coupled with MS) for convenient proteome profiling of 50-1000 mammalian cells. Building upon our recent development of SOP-MS for label-free single-cell proteomics at...
ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse) 
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