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MetabolomicsMetabolomic Discrimination of Near Isogenic Low and High Phytate Soybean Seeds
Methodologies for untargeted metabolomic profiling using liquid chromatography-mass spectrometry (LC-MS) were developed and applied to lipid-depleted methanolic extracts of soybeans seeds obtained from four near isogenic soybean lines (NILs) that differed in phytate content. The four lines differed ...
2016-02-04 | MTBLS120 | MetaboLights
TranscriptomicsExpression data from the seed coats of black (iRT) and brown (irT) soybean variant for alleles of the R locus
The seed coat of black (iRT) soybean with the dominant R allele begins to accumulate cyanic pigments at the transition stage of seed development (300 – 400 mg fresh seed weight), whereas the brown (irT) nearly-isogenic seed coat with the recessive r allele lacks cyanic pigments at all stages of seed...
ORGANISM(S): Glycine max 
2011-08-22 | E-GEOD-26208 | biostudies-arrayexpress
TranscriptomicsFlux of transcript patterns during soybean seed development
Background: To understand gene expression networks leading to functional properties of the soybean seed, we have undertaken a detailed examination of soybean seed development during the stages of major accumulation of oils, proteins, and starches, as well as the desiccating and mature stages, using ...
ORGANISM(S): Glycine max 
2010-05-05 | E-GEOD-18620 | biostudies-arrayexpress
TranscriptomicsIdentification of soybean seed developmental stage specific and tissue specific miRNA targets by degradome sequencing .
Five degradome libraries were constructed from three different seed developmental stages. Separate degradome libraries were constructed for seed coat and cotyledons to identify the tissue specific miRNAs and their potential targets. Sequencing and analysis of degradome libraries gives identification...
ORGANISM(S): Glycine max 
2012-08-07 | E-GEOD-34433 | biostudies-arrayexpress
TranscriptomicsGene Expression Changes in the Development of the Soybean Seed-Early Maturation Stage
We investigated soybean seed development because (1) soybean seeds are a major source of food and fuel, (2) soybean seeds have been an excellent system for studying the basic processes controlling seed development for over three decades, and (3) new soybean genomic resources, including the sequence ...
ORGANISM(S): Glycine max 
2013-04-17 | E-GEOD-46096 | biostudies-arrayexpress
TranscriptomicsRNA-Seq Profiling of a Defective Seed Coat Mutation in Glycine max Reveals Differential Expression of Proline Rich and Other Cell Wall Protein Transcripts
The plant cell wall performs a number of essential functions including providing shape to many different cell types and serving as a defense against potential pathogens. The net pattern mutation creates breaks in the seed coat of soybean (Glycine max) because of ruptured cell walls. Using RNA-Seq, w...
ORGANISM(S): Glycine max 
2014-05-16 | E-GEOD-54903 | biostudies-arrayexpress
TranscriptomicsRNA-seq of soybean lines varying in oil composition and content
Genetic/genome diversity underlying variation in seed oil composition and content among soybean varieties is largely attributed to differences in transcript sequences and/or transcript accumulation of oil production related genes in seeds. Discovery and analysis of sequence and expression variations...
ORGANISM(S): Glycine max 
2016-01-13 | E-GEOD-56297 | biostudies-arrayexpress
TranscriptomicsGenome-wide transcriptome analyses of developing seeds from low and normal phytic acid soybean lines
Purpose: Comprehensive comparison of gene-expression profiles between 3mlpa (mutant with low phytic acid) and 3MWT (non-mutant with normal phyic acid) soybean lines (Glycine max) at five stages of seed development using RNA-Seq approaches. Methods: mRNA sequencing reads (101SE) were generated in tri...
ORGANISM(S): Glycine max 
2016-01-15 | E-GEOD-75575 | biostudies-arrayexpress
TranscriptomicsTranscriptome changes in soybean seeds resulting from RNAi suppression of seed storage proteins
We report the changes in transcriptome that results from RNAi suppression of 7S and 11S storage protein synthesis in soybean seeds, Proteomic results show that other proteins compensate for the storage protein shortfall. The transcriptome analysis show numerous changes result from suppressing storag...
ORGANISM(S): Glycine max 
2010-05-01 | E-GEOD-21116 | biostudies-arrayexpress
ProteomicsShotgun proteomic analysis of embryonic axis of soybean seeds during germination under salt stress
Salinity is one of the main environmental stresses worldwide limiting soybean growth and yield. Seed imbibition and radical emergence are generally less affected by salinity in soybean. Towards unraveling the mechanisms underlying salt tolerance in soybean at germination stage, a comprehensive quant...
ORGANISM(S): Glycine Max (ncbitaxon:3847) 
2016-08-15 | MSV000080044 | MassIVE
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