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TranscriptomicsSpliceosome-Mediated-Decay (SMD) regulates expression of non-intronic genes in budding yeast
Purpose: The goals of this study were to determine whether the spliceosome interacts with non-intronic mRNAs Methods: RNAseq was performed on RNA that immunoprecipitated with the yeast SMD1 protein. Tandem-affinity-purified RNAs were extracted and RNAseq libraries were generated using the EpiCentre...
ORGANISM(S): Saccharomyces cerevisiae 
2013-10-11 | E-GEOD-49966 | biostudies-arrayexpress
TranscriptomicsSpliceosome iCLIP
The studies of spliceosomal interactions are challenging due to their dynamic nature. Here we developed spliceosome iCLIP, which immunoprecipitates SmB along with snRNPs and auxiliary RNA binding proteins (RBPs) to simultaneously map the spliceosomal binding to human snRNAs and pre-mRNAs. This ident...
ORGANISM(S): Homo sapiens 
2019-08-30 | E-MTAB-6950 | biostudies-arrayexpress
TranscriptomicsSpliceosome iCLIP in multiple cell lines
The studies of spliceosomal interactions are challenging due to their dynamic nature. Here we developed spliceosome iCLIP, which immunoprecipitates SmB along with snRNPs and auxiliary RNA binding proteins (RBPs) to simultaneously map the spliceosomal binding to human snRNAs and pre-mRNAs. This ident...
ORGANISM(S): Homo sapiens 
2019-08-03 | E-MTAB-8182 | biostudies-arrayexpress
ProteomicsA Mass Spectrometry Approach Reveals a New Toxic Role For Alzheimer’s Disease A? Peptide: SPLICEOSOME IMPAIRMENT
Alzheimer’s disease is the most common form of dementia, and its prevalence increases exponentially with age. The patients affected gradually lose cognitive function, control over their sense of orientation, their emotions, and other aspects of behavior. Thirty-five million people are now considered...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-04-27 | MSV000081021 | MassIVE
TranscriptomicsSKIP Is a Component of the Spliceosome Linking Alternative Splicing and the Circadian Clock in Arabidopsis
Circadian clocks generate endogenous rhythms in most organisms from cyanobacteria to humans and facilitate entrainment to environmental diurnal cycles, thus conferring a fitness advantage. Both transcriptional and posttranslational mechanisms are prominent in the basic network architecture of circad...
ORGANISM(S): Arabidopsis thaliana 
2012-12-13 | E-GEOD-32216 | biostudies-arrayexpress
GenomicsSpliceosome iCLIP
Spliceosome iCLIP
PRJEB27592 | ENA
TranscriptomicsAberrant splicing of U12-type introns is the hallmark of ZRSR2 mutant myelodysplastic syndrome
Somatic mutations in the spliceosome gene ZRSR2 (located on the X chromosome) are associated with myelodysplastic syndrome (MDS). ZRSR2 is involved in the recognition of 3' splice site during the early stages of spliceosome assembly; however, its precise role in RNA splicing has remained unclear. He...
ORGANISM(S): Homo sapiens 
2015-01-15 | E-GEOD-63816 | biostudies-arrayexpress
ProteomicsMechanism of spliceosome termination
After removing an intron from pre-mRNA, the spliceosome becomes trapped in a non productive complex bound to the excised intron. The termination of this complex is critical for spliceosome recycling and intron decay, but the underlying mechanism remains unknown. Here, we present cryo-electron micros...
ORGANISM(S): Homo sapiens (Human) 
2026-08-17 | PXD078004 | Pride
Spliceosome 
GenomicsA splicing-dependent transcriptional checkpoint associated with pre-spliceosome formation
There is good evidence for functional interactions between splicing and transcription in eukaryotes, but how and why these processes are coupled remain unknown. Prp5 is an RNA-stimulated ATPase required for pre-spliceosome formation in yeast. We demonstrate through in vivo RNA labelling that, in add...
ORGANISM(S): Saccharomyces cerevisiae 
2014-02-19 | E-GEOD-53647 | biostudies-arrayexpress
TranscriptomicsOncogenic MYC induces a dependency on the spliceosome in human cancer
c-MYC (MYC) overexpression or hyperactivation is one of the most common drivers of human cancer. Despite intensive study, the MYC oncogene remains recalcitrant to therapeutic inhibition. Like other classic oncogenes, hyperactivation of MYC leads to collateral stresses onto cancer cells, suggesting t...
ORGANISM(S): Homo sapiens 
2015-09-03 | E-GEOD-66182 | biostudies-arrayexpress
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