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ProteomicsA cyanobacterial screening platform for Rubisco mutant variants
Rubisco is the main entry point of inorganic carbon into the biosphere and a central player in the global carbon system. Its relatively low catalytic constant as well as its tendency to also accept O2 as a substrate have made it a common target of enzyme engineering. We have developed an enzyme engi...
ORGANISM(S): Synechocystis sp. (strain PCC 6803 / Kazusa) 
2025-08-04 | PXD059631 | Pride
Prosit Dia Carbon fixation Rubisco Cyanobacteria Encyclopedia Synechocystis pcc6803 
ProteomicsFunctional modes of Photosystem II under photomixotrophy in distinct variants of Synechocystis sp. PCC 6803
The regulation of photosynthetic electron transport during photomixotrophic growth in cyanobacteria remains incompletely understood. In this study, we characterized four wild-type strains (WT1–WT4) of Synechocystis sp. PCC 6803 and observed distinct strain-specific differences in Photosystem II (PSI...
ORGANISM(S): Synechocystis sp. (strain PCC 6803 / Kazusa) Bacteria 
2026-01-02 | PXD066779 | Pride
Cyanobacteria Dia Synechocystis pcc6803 Orbitrap Photosystem Glucose 
ProteomicsMetabolite interactions in the bacterial Calvin cycle and implications for flux regulation
Metabolite-level regulation of enzyme activity is important for microbes to cope with environmental shifts. Knowledge of such regulations can also guide strain engineering to improve industrial phenotypes. Recently developed chemoproteomics workflows allow for genome-wide detection of metabolite-pro...
ORGANISM(S): Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2) Hydrogenophaga pseudoflava Synechocystis sp. (strain PCC 6803 / Kazusa) Bacteria Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha) 
2023-08-23 | PXD044412 | Pride
Cupriavidus necator Cyanobacteria Prosit Synechococcus sp. pcc7942 Interaction proteomics Encyclopedia Hydrogenophaga pseudoflava Lip-smap Carbon fixation Synechocystis sp. pcc6803 Ralstonia eutropha 
ProteomicsThe fitness landscape of a Form II Rubisco in a photosynthetic bacterium guides engineering of oxygen tolerance
A pooled library of Synechocystis PCC6803 mutants expressing different RuBisCO variants were grown in turbidostats in different gas conditions and the proteome was analyzed by DIA LC/MS proteomics with a Q Exactive HF mass spectrometer after 0, 4 and 8 generations of growth
ORGANISM(S): Synechocystis sp. PCC 6803 
2026-07-16 | PXD071825 | Pride
Synechocystis Machine learning Cyanobacteria Rubisco Carbon fixation Enzyme engineering 
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