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ProteomicsIdentification of novel constituents of ALIS from macrophage lysates (LPS+/-), LC MS/MS
Macrophages assemble aggresome-like induced structures or ALIS upon LPS or E. coli mediated TLR4 stimulation. These structures are positive for p62, LC3 and ubiquitinated proteins, form irrespective of autophagy, and persist within the cell system up to 48h. However, their composition is not well kn...
ORGANISM(S): Mus musculus (Mouse) 
2024-05-23 | PXD031163 | Pride
Lps Lc ms/ms Raw264.7 Alis Macrophages 
ProteomicsQuantitative proteomic analysis reveals JMJD6 and DNAJB11 as endogenous substrates of E3 ligase RFFL
Immunoprecipitation experiments using anti-RFFL antibody. Samples were loaded in technical duplicates in EvoTips, and chromatographic separation was done on an in-house column and the 30 samples per day (SPD) method on Evosep One system (Evosep). Peptides were detected and fragmented using timsTOF P...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2025-01-16 | MSV000096868 | MassIVE
ProteomicsQuantitative proteomic analysis reveals JMJD6 and DNAJB11 as endogenous substrates of E3 ligase RFFL
A549 cells were washed with ice-cold PBS three times and harvested using mechanical scraping. Cell pellets were processed as described previously (Marathe et al., 2021). Briefly, the pellet was re-solubilized in Urea-Thiourea Buffer (6M Urea, 2M Thio-urea in Tris-Cl) containing protease inhibitor co...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2025-01-15 | MSV000096861 | MassIVE
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