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ProteomicsComparing SILAC- and stable isotope dimethyl-labeling approaches for quantitative proteomics.
Comparison of SILAC and reductive dimethyl labeling; also applied both methods to affinity enrichment experiments with and without fractionation.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-08-29 | MSV000082868 | MassIVE
ProteomicsQuantitative nascent proteome profiling by pulse labeling of O-propargyl-puromycin and stable isotope labeled amino acids
Recently, a clickable puromycin analog, O-propargyl-puromycin (OPP), was developed and applied to label C-termini of nascent polypeptide chains (NPCs), followed by a proteomic analysis of NPCs that can be affinity purified via click reaction. Despite its advantage, the affinity purification of NPCs ...
ORGANISM(S): Homo Sapiens (human) 
2020-09-17 | PXD019459 | JPOST Repository
ProteomicsDiscovery of peptide biomarkers in male Eupolyphaga sinensis Walker (Tubiechong) by dimethyl labeling-based quantitative peptidomics analysis
Eupolyphaga sinensis Walker is a traditional Chinese medicinal insect. However, the peptide composition differences between male and female ES, which may underlie their distinct medicinal efficacies, remain largely unexplored. In this study, we performed a quantitative peptidomics analysis using sta...
ORGANISM(S): Eupolyphaga sinensis 
2026-09-07 | PXD080333 | Pride
Eupolyphaga sinensis peptide biomarkers dimethyl labeling Lc-ms/ms 
ProteomicsLarge scale qualitative and quantitative profiling of tyrosine phosphorylation using a combination of phosphopeptide immuno-affinity purification and stable isotope dimethyl labeling
Triplex stable isotope dimethyl labeling of phosphotyrosine peptides after EGF stimulation
ORGANISM(S): Homo sapiens (Human) 
2010-07-09 | PRD000123 | Pride
ProteomicsEstablishment of dimethyl labeling-based quantitative acetylproteomics in Arabidopsis
Protein acetylation, one of many types of post-translational modifications (PTMs), is involved in a variety of biological and cellular processes. In the present study, we applied both CsCl density gradient (CDG) centrifugation-based protein fractionation and a dimethyl-labelling-based 4C quantitativ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-03-14 | PXD006177 | Pride
Cscl density gradient (cdg) centrifugation Stable isotope dimethyl labelling Arabidopsis Squa-d Acetylproteomics 4c proteomic workflow Quantitative ptm proteomics 
ProteomicsDimethyl labeling-based quantitative PTM proteomic analysis of drought-regulated phosphoproteomes in soybean drought-tolerant cultivar
The isotopic dimethyl labeling-based quantitative post-translational modification proteomics was applied to study the phosphoproteomic changes associated with the drought responses of two contrasting soybean cultivars. A total of 9,457 non-redundant, unambiguous, label-independent and repeatable pho...
ORGANISM(S): Glycine max 
2023-05-10 | PXD022806 | Pride
Dimethyl labelling Quantitative ptm proteomics Orbitrap fusion lumos Phosphoproteomics Glycine max Overly post-translationally modified protein (opp) Soybean drought-tolerant cultivar 
ProteomicsDimethyl labeling-based quantitative PTM proteomic analysis of drought-regulated phosphoproteomes in soybean drought-tolerant cultivar
The isotopic dimethyl labeling-based quantitative post-translational modification proteomics was applied to study the phosphoproteomic changes associated with the drought responses of two contrasting soybean cultivars. A total of 9,457 non-redundant, unambiguous, label-independent and repeatable pho...
ORGANISM(S): Glycine max 
2021-09-09 | PXD022794 | Pride
Dimethyl labelling Quantitative ptm proteomics Orbitrap fusion lumos Phosphoproteomics Glycine max Overly post-translationally modified protein (opp) Soybean drought-tolerant cultivar 
UnknownIn-depth qualitative and quantitative profiling of tyrosine phosphorylation using a combination of phosphopeptide immunoaffinity purification and stable isotope dimethyl labeling.
Not available
S-EPMC2808269 | biostudies-literature
UnknownLC-MS Quantification of Site-Specific Phosphorylation Degree by Stable-Isotope Dimethyl Labeling Coupled with Phosphatase Dephosphorylation.
Not available
S-EPMC7697701 | biostudies-literature
UnknownFeasibility of Utilizing Stable-Isotope Dimethyl Labeling in Liquid Chromatography⁻Tandem Mass Spectrometry-Based Determination for Food Allergens-Case of Kiwifruit.
Not available
S-EPMC6571768 | biostudies-literature
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