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MetabolomicsStable Isotope–Assisted Plant Metabolomics: Combination of Global and Tracer-Based Labeling for Enhanced Untargeted Profiling and Compound Annotation
Untargeted approaches and thus biological interpretation of metabolomics results are still hampered by the reliable assignment of the global metabolome as well as classification and (putative) identification of metabolites. In this work we present an liquid chromatography-mass spectrometry (LC-MS)-b...
2020-02-09 | MTBLS1217 | MetaboLights
MetabolomicsProfiling aromatic amino acid metabolome in the human gut microbiota reveals Clostridioides difficile specific N-acyl amino acids

Owing to its compositional and chemical complexity, much of the gut microbiota metabolome remains poorly characterized. Aromatic amino acids (AAAs) have a history of being privileged substrates for the biosynthesis of diverse bioactive metabolites and thus represent a potentially rich source of b...

2026-07-23 | MTBLS13404 | MetaboLights
MetabolomicsIsotope tracing-based metabolite identification for mass spectrometry metabolomics

Modern mass spectrometry-based metabolomics is a key technology for biomedicine, enabling discovery and quantification of a wide array of biomolecules critical for human physiology. Yet, only a fraction of human metabolites have been structurally determined, and the majority of features in typica...

2025-08-15 | MTBLS12861 | MetaboLights
ProteomicsPlant SILAC: Stable-Isotope Labelling with Amino Acids of Arabidopsis Seedlings for Quantitative Proteomics.
Stable Isotope Labelling by Amino acids in Cell culture (SILAC) is a powerful technique for comparative quantitative proteomics, which has recently been applied to a number of different eukaryotic organisms. Inefficient incorporation of labelled amino acids in cell cultures of Arabidopsis thaliana h...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2016-08-15 | MSV000080043 | MassIVE
ProteomicsQuantitative nascent proteome profiling by pulse labeling of O-propargyl-puromycin and stable isotope labeled amino acids
Recently, a clickable puromycin analog, O-propargyl-puromycin (OPP), was developed and applied to label C-termini of nascent polypeptide chains (NPCs), followed by a proteomic analysis of NPCs that can be affinity purified via click reaction. Despite its advantage, the affinity purification of NPCs ...
ORGANISM(S): Homo Sapiens (human) 
2020-09-17 | PXD019459 | JPOST Repository
ProteomicsSILAC-Based Proteomics of Primary Human Kidney Cells treated with Sex Hormones.
Male sex predispose to many kidney diseases. Considering that androgens exert deleterious effects in a variety of cell types within kidney, we hypothesized that dihydrotestosterone (DHT) would impair the biology of the renal tubular cell by inducing changes in the proteome. We employed stable isotop...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-31 | MSV000080854 | MassIVE
ProteomicsComparing SILAC- and stable isotope dimethyl-labeling approaches for quantitative proteomics.
Comparison of SILAC and reductive dimethyl labeling; also applied both methods to affinity enrichment experiments with and without fractionation.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-08-29 | MSV000082868 | MassIVE
MetabolomicsPhotorespiration is linked to DNA methylation by formate as one-carbon source.
DNA methylation is a key epigenetic modification regulating genome organization, stability, and gene expression. Stable DNA methylation critically relies on methyl groups provided through folate-mediated one-carbon (C1) metabolism, yet the origin and regulation of C1 supply remain elusive. Here we d...
2026-01-06 | MTBLS13444 | MetaboLights
Proteomics,Multiomicsp53-regulated networks of protein, mRNA, miRNA and lncRNA expression revealed by integrated pSILAC and NGS analyses
We determined the effect of p53 activation on de novo protein synthesis using quantitative proteomics of newly synthesized proteins (pulsed stable isotope labeling with amino acids in cell culture, pSILAC) in combination with mRNA and non-coding RNA expression analyses by next generation sequencing ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-05-24 | MSV000079767 | MassIVE
ProteomicsSILAC-Tp (12 importins)
Importin-beta family proteins are nucleocytoplasmic transport receptors (NTRs), and they transport most nuclear proteins as their cargoes into and out of the nuclei. Human cells have 20 species of importin-beta family NTRs, of which 10 (importin-beta, transportin-1, -2, -SR, importin-4, -5, -7, -8, ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-30 | MSV000080815 | MassIVE
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