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MetabolomicsInsights into plant lipid metabolism using stable isotopes and high resolution mass spectrometry
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ORGANISM(S): Camelina Sativa 
ST002239 | MetabolomicsWorkbench
MetabolomicsStable Isotope-Assisted Metabolomics for Deciphering Xenobiotic Metabolism in Mammalian Cell Culture
Xenobiotics are ubiquitous in the environment and modified abundantly in the human body by phase I and II metabolism. Liquid chromatography coupled to high resolution mass spectrometry is a powerful tool to investigate these biotransformation products. Here, we present a workflow based on stable iso...
2020-04-14 | MTBLS1358 | MetaboLights
MetabolomicsgeoRge: A Computational Tool To Detect the Presence of Stable Isotope Labeling in LC/MS-Based Untargeted Metabolomics
Studying the flow of chemical moieties through the complex set of metabolic reactions that happen in the cell is essential to understanding the alterations in homeostasis that occur in disease. Recently, LC/MS-based untargeted metabolomics and isotopically labeled metabolites have been used to facil...
2015-09-15 | MTBLS213 | MetaboLights
MetabolomicsStable isotope labelling of Toxoplasma gondii under iron and copper restriction

Toxoplasma uses metal nutrients like iron and copper for vital functions in their energy metabolism. Here, we have used stable isotope labelling to track the incorporation of glucose and glutamine into metabolic pathways, primarily focusing on central carbon metabolism, when these metals are disr...

2026-03-18 | MTBLS13492 | MetaboLights
MetabolomicsStable Isotope–Assisted Plant Metabolomics: Combination of Global and Tracer-Based Labeling for Enhanced Untargeted Profiling and Compound Annotation
Untargeted approaches and thus biological interpretation of metabolomics results are still hampered by the reliable assignment of the global metabolome as well as classification and (putative) identification of metabolites. In this work we present an liquid chromatography-mass spectrometry (LC-MS)-b...
2020-02-09 | MTBLS1217 | MetaboLights
MetabolomicsGlobal Stable-isotope Tracing Metabolomics Reveals System-wide Metabolic Alternations in Aging Drosophila

System-wide metabolic homeostasis is crucial for maintaining physiological functions of living organisms. Stable-isotope tracing metabolomics allows to unravel metabolic activity quantitatively by measuring the isotopically labeled metabolites, but has been largely restricted by coverage. Yet, de...

2022-06-16 | MTBLS3322 | MetaboLights
MetabolomicsElucidation of xenoestrogen metabolism by non-targeted, stable isotope-assisted mass spectrometry in breast cancer cells

Environmental exposure to xenoestrogens, i.e., chemicals that imitate the hormone 17β-estradiol, has the potential to influence hormone homeostasis and action. Detailed knowledge of xenobiotic biotransformation processes in cell models is key when transferring knowledge learned from in vitro<...

2023-10-26 | MTBLS2716 | MetaboLights
MetabolomicsStable isotope resolved analysis of HUVEC Cells under normaxia and hypoxia
In order to analyze HUVECs under normoxia and hypoxia, cells were initially deprived of excess nutrients and hormones by maintaining them overnight in a restricted medium with only 2% fetal bovine serum, and incubated for 40 hours in the presence of the tracer [1,2-13C2]-glucose until sample collect...
2017-12-18 | MTBLS412 | MetaboLights
MetabolomicsPositional Enrichment by Proton Analysis (PEPA): a One-Dimensional 1H-NMR Approach for 13C Stable Isotope Tracer Studies in Metabolomics
A novel metabolomics approach for NMR-based stable isotope tracer studies called PEPA is presented, and its performance validated using human cancer cells. PEPA detects the position of carbon label in isotopically enriched metabolites and quantifies fractional enrichment by indirect determination of...
2017-02-23 | MTBLS247 | MetaboLights
ProteomicsCombined Metabolic and Chemical (CoMetChem) labeling using stable isotopes
Histone acetylation is an important, reversible posttranslational protein modification and hallmark of epigenetic regulation. However, little is known about the dynamics of reversible hisotne acetylation, due to the lack of analytical methods that can capture site-specific acetylation and deacetylat...
ORGANISM(S): Mus musculus (Mouse) 
2021-11-02 | PXD027338 | Pride
Lc-msms Stable isotopes Acetylation Histones 
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