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TranscriptomicsGene expression profiling of Lgr5 positive cells within intestinal adenomas
The generation of the Lgr5_EGFP_ires_CreERT2 knock-in mouse allows marking of Lgr5 positive cells of different tissues. Here we use these mice to sort Lgr5 positive cells and their daughter cells form intestinal adenomas and describe the expression profile of these two cell populations. Intestinal a...
ORGANISM(S): Mus musculus 
2012-08-12 | E-GEOD-37926 | biostudies-arrayexpress
MetabolomicsMetabolic Drug Response Phenotyping in Colorectal Cancer Organoids by LC-QTOF-MS
As metabolic rewiring is crucial for cancer cell proliferation, metabolic phenotyping of patient-derived organoids is desirable to identify drug-induced changes and trace metabolic vulnerabilities of tumor subtypes. We established a novel protocol for metabolomic and lipidomic profiling of colorecta...
2021-10-12 | MTBLS2130 | MetaboLights
TranscriptomicsTranscription profiling of mouse intestinal cells expressing Lgr5 at high or low levels or with Ascl2 conditionally knocked out shows Ascl2 controls intestinal stem cell fate
The small intestinal epithelium is the most rapidly self-renewing tissue of mammals. Proliferative cells are confined to crypts, while differentiated cell types predominantly occupy the villi. We recently demonstrated the existence of a long-lived pool of cycling stem cells defined by Lgr5 expressio...
ORGANISM(S): Mus musculus 
2009-03-25 | E-GEOD-14201 | biostudies-arrayexpress
TranscriptomicsChromatin immunoprecipitation of mouse pancreatic beta-cells.
To gain insights into how pancreatic beta-cells are programmed in vivo, we profiled key histone methylations (H3K4/K27me3) in embryonic stem cells, multipotent progenitors of the nascent embryonic pancreas, purified beta-cells, and 10 other adult tissues (all under normal, untreated conditions). For...
ORGANISM(S): Mus musculus 
2010-03-19 | E-TABM-906 | biostudies-arrayexpress
TranscriptomicsCOMPARATIVE TRANSCRIPTOMIC AND PROTEOMIC ANALYSIS OF LGR5+ve STEM CELLS AND THEIR DAUGHTERS
This SuperSeries is composed of the following subset Series: GSE23672: COMPARATIVE TRANSCRIPTOMIC AND PROTEOMIC ANALYSIS OF LGR5+ve STEM CELLS AND THEIR DAUGHTERS (AGILENT ARRAYS) GSE33948: COMPARATIVE TRANSCRIPTOMIC AND PROTEOMIC ANALYSIS OF LGR5+ve STEM CELLS AND THEIR DAUGHTERS (AFFYMETRIX ARRAYS...
ORGANISM(S): Mus musculus 
2012-08-12 | E-GEOD-33949 | biostudies-arrayexpress
TranscriptomicsCOMPARATIVE TRANSCRIPTOMIC AND PROTEOMIC ANALYSIS OF LGR5+ve STEM CELLS AND THEIR DAUGHTERS (AGILENT ARRAYS)
The identification of Lgr5 as an intestinal stem cell marker has made it possible to isolate and study primary intestinal stem cells. Applying quantitative mass spectrometry as well as transcriptomic analysis, we profiled the protein and gene changes between FACS-sorted Lgr5+ve stem cells and their ...
ORGANISM(S): Mus musculus 
2012-08-12 | E-GEOD-23672 | biostudies-arrayexpress
TranscriptomicsPaneth cells constitute the niche for Lgr5 stem cells in intestinal crypts
Homeostasis of self-renewing small intestinal crypts results from neutral competition between Lgr5 stem cells, small cycling cells located at crypt bottoms1, 2. Lgr5 stem cells are interspersed between terminally differentiated Paneth cells, that are known to produce bactericidal products such as ly...
ORGANISM(S): Mus musculus 
2010-11-29 | E-GEOD-25109 | biostudies-arrayexpress
TranscriptomicsLong-term in vitro expansion of epithelial organoids from human colon, adenoma, adenocarcinoma and Barrett's Esophagus
Background & Aims: We have recently established long-term culture conditions under which single crypts or stem cells derived from murine small intestine expand over long periods of time. Growing crypts undergo multiple crypt fission events, whilst simultaneously generating villus-like epithelial dom...
ORGANISM(S): Homo sapiens 
2011-12-07 | E-GEOD-28907 | biostudies-arrayexpress
TranscriptomicsBulk RNA-seq of proliferating cells in the mouse stomach corpus epithelium from FUCCI mouse.
The purpose of this bulk RNA-seq experiment is to identify candidate genes that can mark proliferating isthmus progenitors in the mouse stomach corpus epithelium. The cycling isthmus progenitors were isolated based on mVenus expression in FUCCI mouse and used for bulk RNA-seq data generation. The co...
ORGANISM(S): Mus musculus 
2019-08-14 | E-MTAB-6850 | biostudies-arrayexpress
TranscriptomicsGene expression profiling along the intestinal crypt axis.
The identification of Lgr5 as an intestinal stem cell marker has made it possible to isolate and study primary intestinal stem cells. Transcriptional differences between intestinal stem cells can be explored by the use of the Lgr5-eGFP-ires-CreERT2 knock-in mouse. In this mouse model GFP expression ...
ORGANISM(S): Mus musculus 
2012-08-15 | E-GEOD-36497 | biostudies-arrayexpress
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