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TranscriptomicsMapping of nascent RNA in MLL2 F/F and FC/FC MEF cells
Global Run-On has been performed in MLL2 F/F and FC/FC MEF cells. The aim of the experiment is to test how the lack of MLL2 affects nascent RNA transcription. Four samples were analyzed - 2 independent replicates. The experiments were performed using immortalized mouse embryonic fibroblasts (MEF) in...
ORGANISM(S): Mus musculus 
2016-04-13 | E-GEOD-73126 | biostudies-arrayexpress
TranscriptomicsGenome wide profiling of the RNAPII, γH2AX, H3K4Me1, H3K4M2 and H3K4Me3 in MLL2 F/F and FC/FC MEF cells
We investigated the RNAPII and γH2AX occupancy genome wide by ChIP-Seq in MLL2 F/F and FC/FC80 MEF cells. We found that a week after MLL2 excision (FC/FC cells), a group of genes present higher levels of γH2AX and RNAPII near the TSS, as compared to the control (F/F cells). H3K4Me1, H3K4M2 and H3...
ORGANISM(S): Mus musculus 
2016-04-13 | E-GEOD-73124 | biostudies-arrayexpress
GenomicsMutations in histone and other genes in MLL2-impaired human and mouse cells
We used human or mouse MLL2-impaired cells (including a human DSET MLL2 cell line,) as well as their parental controls, and measured mutations in histones and a few other genes that were affected in our gH2AX ChIP-Seq experiments. We found that cells lacking or having impaired MLL2 were presenting s...
ORGANISM(S): Mus musculus 
2016-04-13 | E-GEOD-73128 | biostudies-arrayexpress
TranscriptomicsMll2 is required for H3K4 trimethylation on bivalent promoters in ES cells whereas Mll1 is redundant
Trimethylation of histone 3 lysine 4 (H3K4me3) at promoters of actively transcribed genes is a universal epigenetic mark and a key product of Trithorax-Group action. Here we show that Mll2, one of the six Set1/Trithorax-type H3K4 methyltransferases in mammals, is required for trimethylation of bival...
ORGANISM(S): Mus musculus 
2014-01-20 | E-GEOD-52071 | biostudies-arrayexpress
UnknownArabinofuranose-derived positron-emission tomography radiotracers for detection of pathogenic microorganisms.
Not available
S-EPMC7364301 | biostudies-literature
GenomicsAnalysis of the recruitment of JARID2 via Xist RNA during XCI by ChIP-seq
JARID2 ChIP-seq profile mediated by Xist was investigated using two separate approaches: In the first approach, we use the TX1072 female embryonic stem cell (ESC) line; this is a genetically polymorphic ESC line derived from a mouse with one X-chromosome (X-chr) from the Mus musculus castaneus (Cast...
ORGANISM(S): Mus musculus 
2014-01-23 | E-GEOD-52073 | biostudies-arrayexpress
UnknownCause of Death and Predictors of All-Cause Mortality in Anticoagulated Patients With Nonvalvular Atrial Fibrillation: Data From ROCKET AF.
Not available
S-EPMC4943233 | biostudies-literature
TranscriptomicsA Chromatin Immunoprecipitation Approach Based on BAC Transgenesis
We have devised a technology that enables chromatin immunoprecipitation (ChIP) experiments in cultured human cells by circumventing both the laborious production of protein-specific antibodies and the optimization of crosslinking/immunoprecipitation conditions for each protein. Our approach utilizes...
ORGANISM(S): Homo sapiens 
2010-05-25 | E-GEOD-10845 | biostudies-arrayexpress
UnknownCrystal structure of a wild-type Cre recombinase-loxP synapse reveals a novel spacer conformation suggesting an alternative mechanism for DNA cleavage activation.
Not available
S-EPMC203317 | biostudies-literature
UnknownSecondary (additional) findings from the 100,000 Genomes Project: Disease manifestation, health care outcomes, and costs of disclosure.
Not available
S-EPMC7617895 | biostudies-literature
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