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Metabolomics4-Octyl itaconate as a metabolite derivative inhibits inflammation via alkylation of STING

The Krebs cycle-derived metabolite itaconate, whose production is catalyzed by immune response gene 1 (IRG1), has excellent potential to link immunity and metabolism in activated macrophages through alkylation or competitive inhibition of target proteins. In support of this, our previous study de...

2023-01-15 | MTBLS5877 | MetaboLights
MetabolomicsPP2Cm Dysregulation Is Associated with Impaired BCAA Catabolism and cGAS-STING Activation in Diabetic Kidney Disease
Background and Aims:Branched-chain amino acids (BCAAs) are increasingly implicated in diabetic kidney disease (DKD), yet the role of protein phosphatase 2C mitochondrial (PP2Cm), encoded by PPM1K and regulating BCAA catabolism through the branched-chain α-ketoacid dehydrogenase (BCKDH) complex, rema...
2026-10-09 | MTBLS15967 | MetaboLights
ProteomicsLFQ_TurboID-STING_Tamavidin2-REV
The STING-null RAW264.7 cells expressing TurboID-STING were treated with DMSO or 30 μg/ml DMXAA, and 500 µM biotin was added to the medium. After 1-h incubation, the cells were lysed in 6 M guanidine-HCl containing 100 mM HEPES-NaOH (pH 7.5), 10 mM TCEP, and 40 mM CAA. The lysates were dissolved by ...
ORGANISM(S): Mus Musculus (mouse) 
2020-06-17 | PXD018802 | JPOST Repository
ProteomicsLFQ_TurboID-STING_anti-biotin_antibody
The STING-null RAW264.7 cells expressing TurboID-STING were treated with DMSO or 30 μg/ml DMXAA, and 500 µM biotin was added to the medium. After 1-h incubation, the cells were lysed in 6 M guanidine-HCl containing 100 mM HEPES-NaOH (pH 7.5), 10 mM TCEP, and 40 mM CAA. The lysates were dissolved by ...
ORGANISM(S): Mus Musculus (mouse) 
2020-06-17 | PXD018803 | JPOST Repository
ProteomicsLFQ of streptavidin pulldown from hTBJ1 cells expressing APEX2-STING
Permeabilised hTBJ1 cells expressing APEX2-STING were stimulated with or without 1 μM cGAMP at 10°C and incubated for 1 min in the presence of 1 mM H2O2 and 50 µM biotin phenol. The reaction was then quickly stopped by washing three times with ice-cold 500 mM sodium ascorbate, followed by washing tw...
ORGANISM(S): Homo Sapiens (human) 
2022-12-09 | PXD029735 | JPOST Repository
ProteomicsLFQ of GFP-Trap IP-MS from hTBJ1 cells expressing EGFP-mSTING
Permeabilised hTBJ1 cells expressing EGFP-mSTING were stimulated with or without 1 µM 2’3’-cGAMP at 10°C and cross-linked with 0.1 or 0.5% paraformaldehyde at 4°C for 1 h in three independent experiments. After quenching with 100 mM glycine-NaOH, pH 7.5 at 4°C for 15 min, the cells were lysed in RIP...
ORGANISM(S): Homo Sapiens (human) 
2022-12-09 | PXD029736 | JPOST Repository
ProteomicsPRM of DMXAA-treated Raw264.7 secretome
Raw264.7 cells were cultured in serum-free DMEM containing DMSO or DMXAA for 4 h, and the conditioned media were collected. The precipitated proteins were directly digested with trypsin/Lys-C mix. Three biological replicates of the samples were individually prepared and analyzed by targeted LC-MS/MS...
ORGANISM(S): Mus Musculus (mouse) 
2018-04-04 | PXD009170 | JPOST Repository
ProteomicsLFQ of DMXAA-treated Raw264.7 secretome
Raw264.7 cells were cultured in serum-free DMEM containing DMSO or DMXAA for 4 h, and the conditioned medium were collected. The precipitated proteins were directly digested with trypsin. Three biological replicates of the samples were individually prepared and analyzed by LC-MS/MS. Label-free quant...
ORGANISM(S): Mus Musculus (mouse) 
2018-04-04 | PXD009169 | JPOST Repository
UnknownStimulator of Interferon Genes (STING) Triggers Adipocyte Autophagy.
Not available
S-EPMC10572001 | biostudies-literature
TranscriptomicsInflammation-Driven Carcinogenesis is Mediated through STING [MEFs]
Chronic stimulation of innate immune pathways by microbial agents or damaged tissue is known to promote inflammation-driven tumorigenesis by unclarified mechanisms1-3. Here we demonstrate that mutagenic 7,12-dimethylbenz(a)anthracene (DMBA), etoposide or cisplatin induces nuclear DNA leakage into th...
ORGANISM(S): Mus musculus 
2014-05-31 | E-GEOD-57603 | biostudies-arrayexpress
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