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ProteomicsEscherichia coli sucrose gradient fractionation
We studied localization of E. coli membrane proteome using sucrose gradient fractionation combined with quantitative MS.
ORGANISM(S): Escherichia coli 
2020-02-19 | PXD016403 | Pride
Escherichia coli 
TranscriptomicsSelective mRNA translation during eIF2 phosphorylation induces expression of IBTKalpha
Disruption of protein folding in the endoplasmic reticulum triggers the Unfolded Protein Response (UPR), a transcriptional and translational control network designed to restore protein homeostasis. Central to the UPR is PERK phosphorylation of the alpha subunit of eIF2 (eIF2~P), which represses glob...
ORGANISM(S): Mus musculus 
2014-06-02 | E-GEOD-54581 | biostudies-arrayexpress
TranscriptomicsGlucocorticoid effect on mRNA translation in childhood acute lymphoblastic leukemia
Glucocorticoids (GCs) are a central component in treating childhood acute lymphoblastic leukemia (chALL). They mainly act via regulating gene transcription. However, control of mRNA translation by GC has never been assessed systematically. In our research, T- and precursor B-ALL cells were cultured ...
ORGANISM(S): Homo sapiens 
2013-12-03 | E-GEOD-48680 | biostudies-arrayexpress
TranscriptomicsFine-tuning of human dendritic cells regulation revealed by translational profiling
Dendritic cells (DCs) are the sentinels of the mammalian immune system and they undergo a complex maturation process mediated by activation upon pathogen detection. Recent studies described the analysis of activated DCs by transcriptional profiling, but translation regulation was never taken in acco...
ORGANISM(S): Homo sapiens 
2009-12-30 | E-GEOD-14000 | biostudies-arrayexpress
ProteomicsEnrichment of microsomes from Chinese hamster ovary cells by subcellular fractionation for its use in proteomic analysis
Chinese hamster ovary (CHO) cells have been widely employed for production of recombinant proteins (RP) for research and therapeutic purposes. Indeed, this expression system was used for production of most of approved antibodies (84%) in 2015-2018 period. The success of this cell line for RP product...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2020-08-27 | PXD019778 | Pride
Organelles Subcellular fractionation Subcellular proteomics Chinese hamster ovary cells 
TranscriptomicsTranslational activation of developmental mRNAs during neonatal mouse testis development
The sequence of gene regulatory events that drive neonatal germ cell development in the mammalian testis is not yet clear. We assessed changes in mRNA utilization in the neonatal testis at 1 and 4 dpp, times when the testis contains quiescent gonocytes (1 dpp) and proliferating spermatogonia (4 dpp)...
ORGANISM(S): Mus musculus 
2013-07-27 | E-GEOD-49283 | biostudies-arrayexpress
TranscriptomicsEstrogen Coordinates Translation and Transcription Revealing a Role for NRSF in Human Breast Cancer Cells
Analysis of estrogen receptor (ER)-positive MCF7 cell total RNA expression and polysome-assiciated RNA expression following treatment with estradiol (E2) and vehicle (etoh). We used expression microarrays to measure polysome association and total RNA abundance in E2 treated MCF7. These data, along ...
ORGANISM(S): Homo sapiens 
2010-05-07 | E-GEOD-18592 | biostudies-arrayexpress
ProteomicsProteomic Characterization of Post-Mortem Human Brain Tissue Following Ultracentrifugation-based Subcellular Fractionation
Proteomic characterization of human brain tissue is increasingly utilized to identify potential novel biomarker and drug targets for a variety of neurological diseases. In whole tissue studies, results may be driven by changes in the proportion of the largest and most abundant organelles or tissue c...
ORGANISM(S): Homo sapiens (Human) 
2022-05-06 | PXD027456 | Pride
Subcellular fractionation Human brain Label free quantitation Alzheimer's disease 
UnknownDisentangling the Complexity of the Rumen Microbial Diversity Through Fractionation Using a Sucrose Density Gradient.
Not available
S-EPMC8297521 | biostudies-literature
TranscriptomicsContrast of poly-ribosomal mRNA in germina vesicle (GV) and metaphase II (MII) bovine oocytes.
Poly-ribosomal mRNA from pools of 75 bovine oocytes at the germinal vesicle(GV) and metaphase II(MII) stages were isolated by sucrose fractionation then compared through microarray hybridization. 3 biological replicates from germinal vesicle(GV) and metaphase II(MII) bovine oocytes were hybridized a...
ORGANISM(S): Bos taurus 
2014-09-09 | E-GEOD-56603 | biostudies-arrayexpress
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