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Pseudomonas aeruginosa was prepared and adjusted to an initial bacterial number of OD600 = 1.0 in Tryptic soy broth with or without 5 μg/mL SaEVs. After incubation at 37°C for 24 h, the bacterial cells were collected by centrifugation at 1,100 x g, 4°C for 15 min, followed by washing twice with phos...
ORGANISM(S): Pseudomonas Aeruginosa 
Staphylococcus aureus strain ATCC 1718 was inoculated at 0.5% in 400 mL of Brain Heart Infusion broth for 8 h under aerobic conditions. The supernatant was collected by centrifugation twice at 5,000 x g, 4°C for 20 min, filtrated through a 0.45 µm filter to remove all remaining bacterial cells. S. a...
ORGANISM(S): Staphylococcus Aureus 
PaOMVs in the culture supernatant of P. aeruginosa were harvested by ultracentrifugation at 100,000 × g, 4℃ for 90 min using a Himac CP80WX Preparative Ultracentrifuge (HITACHI, Tokyo, Japan). After removal of the supernatant, the pellet was washed twice and suspended in ice-cold sterile phosphate-b...
ORGANISM(S): Pseudomonas Aeruginosa 
For proteomic analysis of PaOMV-treated S. aureus, S. aureus (5 × 103 CFU/mL) was incubated with and without 1 µg/mL PaOMVs for 6 h at 37℃ under static condition and proteomic analysis was performed as described above. The identified proteins were searched against S. aureus ATCC1718 (NCBI Genebank: ...
ORGANISM(S): Staphylococcus Aureus 
Acinetobacter baumannii were cultured in Brain Heart Infusion medium (BD Bioscience). For normoxic condition, the cultures were shaken at 125 rpm for 24 h, whereas for hypoxic condition, the cultures were placed under static condition for 48 h. Outer membrane vesicles from A. baumannii (AbOMVs) in t...
ORGANISM(S): Acinetobacter Baumannii 
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