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Proteomic analysis of Staphylococcus aureus after treating with extracellular membrane vesicles from Pseudomonas aeruginosa (PaOMVs)

ABSTRACT: For proteomic analysis of PaOMV-treated S. aureus, S. aureus (5 × 103 CFU/mL) was incubated with and without 1 µg/mL PaOMVs for 6 h at 37℃ under static condition and proteomic analysis was performed as described above. The identified proteins were searched against S. aureus ATCC1718 (NCBI Genebank: NC_007795.1). In comparison with non-PaOMV treatment, differential protein expression in PaOMV-treated S. aureus was assessed by quantitative proteomics using SWATH analysis.

ORGANISM(S): Staphylococcus Aureus

SUBMITTER: Krisana Asano

PROVIDER: PXD036381 | JPOST Repository | Mon May 08 00:00:00 BST 2023

REPOSITORIES: jPOST

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Dataset's files

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			Action	DRS
	210623_Sa+PaOMVs1_IDA.wiff	Wiff
	210623_Sa+PaOMVs1_IDA.wiff.scan	Wiff
	210623_Sa+PaOMVs1_SWATH.wiff	Wiff
	210623_Sa+PaOMVs1_SWATH.wiff.scan	Wiff
	210623_Sa+PaOMVs2_IDA.wiff	Wiff

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Publications

Extracellular vesicles of Pseudomonas aeruginosa downregulate pyruvate fermentation enzymes and inhibit the initial growth of Staphylococcus aureus.

Ishiai Takahito T Subsomwong Phawinee P Narita Kouj K Kawai Noriaki N Teng Wei W Suzuki Sachio S Sukchawalit Rojana R Nakane Akio A Asano Krisana K

Current research in microbial sciences 20230420

Staphylococcus aureus and Pseudomonas aeruginosa are well-known opportunistic pathogens that frequently coexist in chronic wounds and cystic fibrosis. The exoproducts of P. aeruginosa have been shown to affect the growth and pathogenicity of S. aureus, but the detailed mechanisms are not well understood. In this study, we investigated the effect of extracellular vesicles from P. aeruginosa (PaEVs) on the growth of S. aureus. We found that PaEVs inhibited ...[more]

PMID: 37131486

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Next Generation Sequencing Facilitates Quantitative Analysis of control of Pseudomonas aeruginosa PAO1 and DATS-treated P. aeruginosa PAO1 Transcriptomes

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Next Generation Sequencing Facilitates Quantitative Analysis of control of Pseudomonas aeruginosa PAO1 and farnesol-treated P. aeruginosa PAO1 Transcriptomes

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