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ProteomicsIdentification of SENP6 targets in HeLa cells by SENP6 SiRNA and MW shift analysis
The SUMO protease SENP6 disassembles SUMO chains and thus SENP6 depletion should lead to the extension of SUMO polymers on substrates. We have used a proteomic method to identify putative SENP6 substrates based on increased apparent molecular weight after SENP6 depletion. This method uses SDS-PAGE f...
ORGANISM(S): Homo sapiens (Human) 
2024-03-01 | PXD036743 | Pride
Sumo senp6 molecular weight 
ProteomicsThe SUMO protease SENP6 regulates the constitutive centromere-associated network by group
Project 1: Identification of SUMOylated proteins which are regulated by the SUMO protease SENP6. SENP6 knockdown was performed by two independent shRNAs and SUMOylated proteins were purified from U2OS expressing His10-tagged SUMO2 by means of Ni-NTA pulldown. Purified SUMOylated proteins were identi...
ORGANISM(S): Homo sapiens (Human) 
2019-09-13 | PXD011963 | Pride
Human osteosacrcoma cell line u2os Chromatin Senp6 
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