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TranscriptomicsPAR-CLIP analysis of YTHDC1, SRSF1, SRSF3, SRSF7, SRSF9 and SRSF10 in human HeLa cells
Label the cells overexpressed Flag tagged YTHDC1, SRSF1, SRSF3, SRSF7, SRSF9 and SRSF10 with 4-SU, the RNA bound by YTHDC1 and SRSF proteins can be got by Flag IP followed by RNA isolation by using the TRIzol (Invitrogen) reagent by following the company manual.the RNA was then used for library prep...
ORGANISM(S): Homo sapiens 
2016-02-17 | E-GEOD-71096 | biostudies-arrayexpress
TranscriptomicsMETTL3 and WTAP
This SuperSeries is composed of the SubSeries listed below. Refer to individual Series
ORGANISM(S): Homo sapiens 
2014-01-16 | E-GEOD-50583 | biostudies-arrayexpress
TranscriptomicsBinding motif analysis of METTL3，WTAP in METTL3，WTAP overexpressed Human 293T cells
label the cells overexpressed Myc tagged METTL3 and Flag tagged WTAP with 4-SU, the RNA bound by METT3,WTAP can be got by Myc or Flag IP followed by RNA isolation by using the TRIzol (Invitrogen) reagent by following the company manual.the RNA was then used for library preparation using a TruSeq™ RN...
ORGANISM(S): Homo sapiens 
2014-01-16 | E-GEOD-50580 | biostudies-arrayexpress
TranscriptomicsTranscriptomics analysis of gene expression in normal and METTL3 or WTAP deficient Human 293T cells
RNA was isolated from and METTL3M-oM-
ORGANISM(S): Homo sapiens 
2014-01-16 | E-GEOD-50582 | biostudies-arrayexpress
Transcriptomicsm6a peak analysis in normal, FTO deficient and five stages of adipogenesis (D-2/0/2/5/10) in Mouse embryo fibroblast 3T3-L1 pre-adipocytes
Total RNA was extracted using TRI® Reagent (Sigma). cDNA was synthesized by RevertAid™ First Strand cDNA Synthesis Kit with Oligo dT primers (K1622, Fermentas) following manufacturer’s recommendations. PCR reactions were carried out on a DNAEngine® Thermal Cycler (PTC-0200G, Bio-Rad) in 25 μl reacti...
ORGANISM(S): Mus musculus 
2015-03-09 | E-GEOD-53244 | biostudies-arrayexpress
TranscriptomicsTranscriptomics analysis of gene expression in normal and Smg6 deficient mouse ES cells
RNA was isolated from control and Smg6 deficient ES cells using the TRIzol (Invitrogen) reagent by following the company manual. For all samples the RNA integrity was checked using an Agilent Bioanalyzer 2100. All samples showed a RIN (RNA integrity number) of higher than 9. Approximately 2.5 M-BM-5...
ORGANISM(S): Mus musculus 
2013-08-14 | E-GEOD-49844 | biostudies-arrayexpress
GenomicsEpigenome analysis of DNA modification 5-hydroxymethylcytosine in normal, abnormal, and globozoospermia sperm genomes
Genome wide of 5-hydroxymethylcytosine profiling of normal and abnormal, and globozoospermia sperm genomes.Two semen samples were collected from a healthy man and a globozoospermia patient who had consulted a physician at ShengJing Hospital of China Medical University. Other semen samples obtained f...
ORGANISM(S): Homo sapiens 
2013-04-18 | E-GEOD-46135 | biostudies-arrayexpress
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