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TranscriptomicsscRNA-seq of T cell differentiation in Artificial Thymic Organoid
We followed the PSC-ATO protocol as previously described in Montel-Hagen et al. 2019, and differentiated from two iPSC lines, HPSI0114i-kolf_2 (Kolf) and HPSI0514i-fiaj_1 (Fiaj), to single positive T cells. The following include scRNA-seq data from intermediate cells sampled at day -14, day -7 (two ...
ORGANISM(S): mixed sample 
2024-02-10 | E-MTAB-12720 | biostudies-arrayexpress
TranscriptomicsMapping the developing human immune system across organs - VDJ-seq
We profiled hematopoietic, lymphoid and peripheral fetal organs to systematically assess the heterogeneity of antigen receptors in immune cell populations across human tissues during development. Single-cell suspensions were obtained from fresh tissue. Cells were either DAPI-CD45+ or DAPI-CD45- FACS...
ORGANISM(S): Homo sapiens 
2022-09-23 | E-MTAB-11388 | biostudies-arrayexpress
TranscriptomicsMapping the developing human immune system across organs - scRNA-seq
We profiled hematopoietic, lymphoid and peripheral fetal organs to systematically assess the heterogeneity of immune cell populations across human tissues during development. Single-cell suspensions were obtained from fresh tissue. Cells were either DAPI-CD45+ or DAPI-CD45- FACS-isolated cells, or u...
ORGANISM(S): Homo sapiens 
2022-05-12 | E-MTAB-11343 | biostudies-arrayexpress
TranscriptomicsA high-resolution single-cell multiomic atlas of the human fetal lung - ATAC-seq
We obtained human embryonic and fetal lungs from 5-22 pcw for scRNAseq and scATACseq analysis. To focus on epithelial differentiation and region specialization, we deeply sampled 15, 18, 20 and 22 pcw lungs and separated proximal and distal regions while leaving lungs at 5, 6, 9 and 11 pcw intact. T...
ORGANISM(S): Homo sapiens 
2022-01-27 | E-MTAB-11266 | biostudies-arrayexpress
TranscriptomicsA high-resolution single-cell multiomic atlas of the human fetal lung - Organoid
Individual TFs were overexpressed in fetal lung tip organoids from a doxycycline-inducible construct for 3 days, and organoids were maintained in the self-renewing (tip cell-promoting) medium throughout to rigorously assay the lineage-determining competence of the TF, followed by scRNA-seq. ASCL1, ...
ORGANISM(S): Homo sapiens 
2022-01-27 | E-MTAB-11267 | biostudies-arrayexpress
TranscriptomicsA high-resolution single-cell multiomic atlas of the human fetal lung - scRNA / GEX / VDJ
We obtained human embryonic and fetal lungs from 5-22 pcw for scRNAseq and scATACseq analysis. To focus on epithelial differentiation and region specialization, we deeply sampled 15, 18, 20 and 22 pcw lungs and separated proximal and distal regions while leaving lungs at 5, 6, 9 and 11 pcw intact. T...
ORGANISM(S): Homo sapiens 
2022-01-27 | E-MTAB-11278 | biostudies-arrayexpress
TranscriptomicsA high-resolution single-cell multiomic atlas of the human fetal lung - Visium
Fetal lung samples at 12–20 post conception week (pcw) from the HDBR, up to 0.5cm3 in size, were embedded in OCT and flash-frozen in dry-ice cooled isopentane. Twelve-micron cryosections were cut onto Visium slides, haematoxylin and eosin stained and imaged at 20X magnification on a Hamamatsu Nanozo...
ORGANISM(S): Homo sapiens 
2022-01-27 | E-MTAB-11265 | biostudies-arrayexpress
TranscriptomicsImmune cells direct epithelial maturation during early human lung development
The adult human lung harbours a significant proportion of immune cells, which are known to play a crucial role in both normal lung homeostasis and pathogenesis. In this work, we collected CD45-enriched human lung cells from 6 to 22 week post conception (pcw) and built a single-cell transcriptome and...
ORGANISM(S): Homo sapiens 
2022-12-09 | E-MTAB-11528 | biostudies-arrayexpress
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