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TranscriptomicsTranscription profiling of mouse wild type and Nkx2.2 mutant embryos at E12.5 and E13.5 to identify a transcripional program that Nkx2.2 regulation in the pancreas
The goal of this experiment was to analyze expression changes in the pancreas at embryonic days 12.5 and 13.5 between wild type and Nkx2.2 null mice. We know that Nkx2.2 is essential for pancreatic endocrine differentiation and development. At these early time points which are critical for endocri...
ORGANISM(S): Mus musculus 
2009-11-05 | E-CBIL-44 | biostudies-arrayexpress
TranscriptomicsRNA Seq analysis of e12.5 mouse pancreatic buds from control and Pdxcre; Gata4fl/fl;Gata6fl/fl; Tom mice
about 250 genes were significantly changed after Gata4 and Gata6 were specifically deleted in the pancreatic progenitor cells 6 pancreatic buds were pooled for the control, and 12 pancreatic buds were pooled for the Pdxcre; Gata4fl/fl; Gata6fl/fl. Libraries were prepared from total RNA (RIN>8) with ...
ORGANISM(S): Mus musculus 
2016-03-08 | E-GEOD-77083 | biostudies-arrayexpress
TranscriptomicsRNAseq analysis of the colon of intestine-specific adult Nkx2.2 mutant mice
Aim: Transcriptional analysis of the colon of adult Nkx2.2flox/flox;Villin-Cre (Nkx2.2int) mice versus control Methods: 2 cm of the colon (as measured after the caecum) of 6 week old control and mutant mice were dissected and total RNA extracted. Libraries were prepared from total RNA (RIN>8) with t...
ORGANISM(S): Mus musculus 
2016-07-28 | E-GEOD-78902 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of mouse wild type and Nkx2.2 knock-out embryos
The goal: Investigate the genes differentlly expressed during pancreas development. The model: The Nkx2.2 null allele was generated using standard homologous recombination in ES cells. The Pgk:NeoR cassette was inserted into a 4.4 Kb deletion of the Nkx2.2 genomic locus, effectively deleting bot...
ORGANISM(S): Mus musculus 
2011-08-27 | E-MTAB-356 | biostudies-arrayexpress
TranscriptomicsFunctional analysis of the Nkx2.2 SD domain: gene expression profiling of E16.5 Nkx2.2 SD mutant pancreata versus wild-type littermate pancreata
The experiment consists of 7 one channel assays for analyzing embryonic pancreas at E16.5. There are three or four replicates per condition. At E16.5, we are looking at differential gene expression across two mouse lines, WT vs Nkx2.2 SD mutants.
ORGANISM(S): Mus musculus 
2015-01-29 | E-MTAB-3349 | biostudies-arrayexpress
TranscriptomicsGene expression profiling of wild-type, Nkx2.2 null, NeuroD null and Nkx2.2/NeuroD double KO E13.5 pancreata
The experiment consist of 16 one channel assays for analyzing embryonic pancreas at E13. There are four replicates per condition. We are looking at differential gene expression across four mouse lines: WT, Nkx2.2, NeuroD and Nkx2.2/NeuroD double mutants at E13.5.
ORGANISM(S): Mus musculus 
2015-02-03 | E-MTAB-3350 | biostudies-arrayexpress
TranscriptomicsRNA sequencing of e15.5 pancreas from Wild Type, Blinc1-/- and Blinc+/- mice.
We report the transcriptome changes that result of the genomic deletion of one or two alleles of an islet-specific long non-coding RNA (Blinc1) in isolated pancreas from e15.5 mouse embryos. Pancreas from e15.5 embryos were dissected and total RNA extracted. Libraries were prepared from total RNA (R...
ORGANISM(S): Mus musculus 
2016-04-06 | E-GEOD-73711 | biostudies-arrayexpress
TranscriptomicsRNAseq analysis of the duodenum of intestine-specific adult TN mutant mice
Aim: Transcriptional analysis of the duodenum of adult Nkx2.2flox/TN;Villin-Cre (TNint) mice versus control Methods: 2 cm of the duodenum (as measured from the stomach) of 6 week old control and mutant mice were dissected and total RNA extracted. Libraries were prepared from total RNA (RIN>8) with t...
ORGANISM(S): Mus musculus 
2016-07-28 | E-GEOD-72764 | biostudies-arrayexpress
TranscriptomicsRNAseq analysis of the duodenum of intestine-specific adult SD mutant mice
Aim: Transcriptional analysis of the duodenum of adult Nkx2.2flox/SD;Villin-Cre (SDint) mice versus control Methods: 2 cm of the duodenum (as measured from the stomach) of 6 week old control and mutant mice were dissected and total RNA extracted. Libraries were prepared from total RNA (RIN>8) with t...
ORGANISM(S): Mus musculus 
2016-07-28 | E-GEOD-72762 | biostudies-arrayexpress
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