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ProteomicsProteomic analysis of Staphylococcus aureus after treating with extracellular membrane vesicles from Pseudomonas aeruginosa (PaOMVs)
For proteomic analysis of PaOMV-treated S. aureus, S. aureus (5 × 103 CFU/mL) was incubated with and without 1 µg/mL PaOMVs for 6 h at 37℃ under static condition and proteomic analysis was performed as described above. The identified proteins were searched against S. aureus ATCC1718 (NCBI Genebank: ...
ORGANISM(S): Staphylococcus Aureus 
2023-05-08 | PXD036381 | JPOST Repository
ProteomicsProteomic analysis of extracellular membrane vesicles from Pseudomonas aeruginosa (PaOMVs)
PaOMVs in the culture supernatant of P. aeruginosa were harvested by ultracentrifugation at 100,000 × g, 4℃ for 90 min using a Himac CP80WX Preparative Ultracentrifuge (HITACHI, Tokyo, Japan). After removal of the supernatant, the pellet was washed twice and suspended in ice-cold sterile phosphate-b...
ORGANISM(S): Pseudomonas Aeruginosa 
2023-07-18 | PXD036377 | JPOST Repository
ProteomicsQuantitative proteomic analysis of extracellular membrane vesicles from Acinetobacter baumannii (AbOMVs) isolated under normoxic and hypoxic conditions.
Acinetobacter baumannii were cultured in Brain Heart Infusion medium (BD Bioscience). For normoxic condition, the cultures were shaken at 125 rpm for 24 h, whereas for hypoxic condition, the cultures were placed under static condition for 48 h. Outer membrane vesicles from A. baumannii (AbOMVs) in t...
ORGANISM(S): Acinetobacter Baumannii 
2023-03-16 | PXD037014 | JPOST Repository
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