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In budding yeast, Set2 catalyzes di- and trimethylation of H3K36 (H3K36me2 and H3K36me3) via an interaction between its SRI domain and C-terminal repeats of RNA polymerase II (Pol2) phosphorylated at Ser2 and Ser5 (CTD-S2,5-P). H3K36me2 recruits the Rpd3S histone deacetylase complex to repress crypt...
ORGANISM(S): Schizosaccharomyces pombe 
The liver and skeletal muscle were collected from 10-week-old wild-type and ob/ob mice fasted for 16 hours. Tissues were frozen immediately and lysed with 0.5 mL of a solution containing 50 mM Tris-HCl pH8.8, 2%SDS, and 7 M urea, and then subjected to ultrasonic treatment with a Bioruptor (Digaenode...
ORGANISM(S): Mus Musculus (mouse) 
Ten-week-old male mice (WT and ob/ob) were starved for 0, 2, 4, 6, 8, 12, 16, and 24 hours and liver samples were collected. Total proteins extracted during sample preparation of metabolomic analysis were subjected to the following phosphoproteomic analysis. As described above, 2 mg of protein solut...
ORGANISM(S): Mus Musculus (mouse) 
This project utilized CRISPR-assisted RNA-protein interaction detection (CARPID) combined with mass spectrometry to identify proteins interacting with the long non-coding RNA (lncRNA) TUG1 in HEK293T cells. TUG1 preferentially interacts with known R-loop-associated proteins, particularly under Campt...
ORGANISM(S): Homo Sapiens (human) 
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