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Gene expression profiling of BMMC from patients with rheumatoid arthritis (RA) vs. osteoarthritis (OA). Bone marrow was obtained from patients with RA (n=9) or OA (n=10). The bone marrow samples from the 10 OA patients are used as controls.
ORGANISM(S): Homo sapiens 
(1) qPCR Gene Expression Data The THP-1 cell line was sub-cloned and one clone (#5) was selected for its ability to differentiate relatively homogeneously in response to phorbol 12-myristate-13-acetate (PMA) (Sigma). THP-1.5 was used for all subsequent experiments. THP-1.5 cells were cultured in ...
ORGANISM(S): Homo sapiens 
Direct cell conversion is now expected to apply to therapeutic purposes. Although that has been succeeded in several cell types, the mechanism or general way to identify the key transcription factors are still unclear. In addition, most of the cases are not completely identical with the target cells...
ORGANISM(S): Homo sapiens 
Myelodysplastic syndromes (MDS) are clonal disorders of hematopoietic progenitors characterized by ineffective hematopoiesis and high propensity to leukemias. Although a number of gene targets have been identified, in many MDS cases, particular genetic targets are unknown. In this study, we performe...
ORGANISM(S): Homo sapiens 
OfMasc-GFP derivatives or GFP were transiently expressed in OfT1C/tet cells (3.2e6 cells) seeded in 10 cm-diameter culture dishes. At two days post transfection, the cells were fixed, collected, and lysed on ice for 10 min in 1 mL of RIPA buffer (20 mM HEPES-KOH, pH 7.5, 1 mM EGTA, 1 mM MgCl2, 150 m...
ORGANISM(S): Cellular Organisms 
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