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ProteomicsiPSC-HDF phosphoproteome
We analyzed iPSC (201B7) and HDF (HDF1388) by global whole phosphoproteome analysis. iTRAQ was used for relative quantification between the two cell types. We did biological triplicate analyses.
ORGANISM(S): Homo Sapiens (human) 
2019-05-14 | PXD012367 | JPOST Repository
ProteomicsiPSC-HDF proteome
We analyzed iPSC (201B7) and HDF (HDF1388) by global whole proteome analysis. iTRAQ was used for relative quantification between the two cell types. We did biological triplicate analyses with technical triplicate analyses.
ORGANISM(S): Homo Sapiens (human) 
2019-05-14 | PXD012368 | JPOST Repository
ProteomicsE.coli and Human proteome
To measure quantification accuracy, we generated two proteome samples of E.coli and human cell lysate. E.coli tryptic peptides were tagged with four different labels of reporter ions (m/z: 114, 115, 116 and 117). These labeled peptides were mixed at the following ratios: 114:115 = 10:1, 114:117 = 5:...
ORGANISM(S): Homo Sapiens (human) Escherichia Coli 
2019-05-14 | PXD011913 | JPOST Repository
ProteomicsDifferences of uniquely identified peptides between ddaPASEF and diaPASEF
Recent advancements in mass spectrometry-based proteomics have made it possible to conduct comprehensive protein analysis. In particular, the emergence of the data-independent acquisition (DIA) method powered by machine learning has significantly improved protein identification effi-ciency. However,...
ORGANISM(S): Homo Sapiens (human) 
2024-12-01 | PXD056754 | JPOST Repository
ProteomicsA stress-reduced passaging technique improves the viability of human pluripotent cells
Xeno-free culture systems have expanded the application of human pluripotent stem cells (PSCs). Here we describe an improved method for the subculture of human PSCs. The revised method significantly facilitated the viability of human PSCs by lowering DNA damage and apoptosis, resulting in more effic...
ORGANISM(S): Homo Sapiens (human) 
2022-01-23 | PXD028731 | JPOST Repository
ProteomicsPost-transcriptionally regulated genes are essential for pluripotent stem cell survival
Here, by combining transcriptome and proteome profiling, we identified 228 post-transcriptionally regulated genes with strict regulation at the protein level in PSCs. Among them, 84 genes were found necessary for the survival of several cell types including PSCs, and 20 genes were identified as spec...
ORGANISM(S): Homo Sapiens (human) 
2022-05-10 | PXD028489 | JPOST Repository
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