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ProteomicsTMTpro-16plex analysis of mouse hippocampus and cortex
Mice brain synaptosomal fractions were homogenized in 6 M guanidine-HCl containing 100 mM HEPES−NaOH (pH 7.5), 10 mM Tris (2-carboxyethyl) phosphine (TCEP), and 40 mM chloroacetamide. After heating and sonication, proteins were purified by methanol−chloroform precipitation and solubilized with 0.1% ...
ORGANISM(S): Mus Musculus (mouse) 
2024-08-20 | PXD052653 | JPOST Repository
ProteomicsTMT-based phosphoproteomics of mouse hippocampus
Brain tissues were homogenized in 6 M guanidine-HCl containing 100 mM HEPES−NaOH (pH 7.5), 10 mM Tris (2-carboxyethyl) phosphine (TCEP), and 40 mM chloroacetamide. After heating and sonication, proteins were purified by methanol−chloroform precipitation and solubilized with 0.1% 21 RapiGest SF in 50...
ORGANISM(S): Mus Musculus (mouse) 
2024-08-20 | PXD052654 | JPOST Repository
ProteomicsTMT-based phosphoproteomics of mouse cortex
Brain tissues were homogenized in 6 M guanidine-HCl containing 100 mM HEPES−NaOH (pH 7.5), 10 mM Tris (2-carboxyethyl) phosphine (TCEP), and 40 mM chloroacetamide. After heating and sonication, proteins were purified by methanol−chloroform precipitation and solubilized with 0.1% 21 RapiGest SF in 50...
ORGANISM(S): Mus Musculus (mouse) 
2024-08-20 | PXD052655 | JPOST Repository
ProteomicsMALDI Quant Fibrinogen
Absolute quantification of protein was performed by MALDI-LTQ using scramble internal standard (sIS) peptide. Mixtures of serial dilutions of fibrinogen peptides (final concentration: 50, 100, 250, 500 and 1000 fmol/μL), equal amounts of corresponding sIS peptides (final concentration: 500 fmol/μL) ...
ORGANISM(S): Homo Sapiens (human) 
2017-04-08 | PXD005772 | JPOST Repository
ProteomicsSchizophrenia brain proteomic analysis
The purpose of the study was to identify altered molecular networks in the prefrontal cortex of schizophrenia patients by comparing protein expression levels in autopsied brains of patients (n=10) and controls (n=10), using aquantitative proteomics.
ORGANISM(S): Homo Sapiens (human) 
2018-01-01 | PXD008227 | JPOST Repository
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