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This study aimed to analyze ductal and squamous marker expression in pancreatic ductal adenocarcinoma (PDAC) cell lines under two-dimensional (2D) and three-dimensional (3D) culture conditions. Eight PDAC cell lines were examined using nanoLC-MS/MS-based proteomics.
ORGANISM(S): Homo Sapiens (human) 
Control or GFP-WIPI2d expressing MEFs were treated with or without 0.5uM Apilimod for one hour, and then cross-linked with 0.1% formaldehyde for ten mins at room temperature. After cross-linking was quenched with 100mM glycine for four mins at room temperature, cells were washed with HEPES saline co...
ORGANISM(S): Mus Musculus (mouse) 
Control or GFP-Rubicon expressing MEFs were treated with or without 0.5uM Apilimod for one hour, and then cross-linked with 0.1% formaldehyde for ten mins at room temperature. After cross-linking was quenched with 100mM glycine for four mins at room temperature, cells were washed with HEPES saline c...
ORGANISM(S): Mus Musculus (mouse) 
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