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Proteomics293T NME4 TAP-MS and TurboID-MS
To identify interacting proteins of NME4 in human cell lines, we using tandem affinity purification followed by mass spectrometry (TAP-MS) in HEK293T cells. We established stable cell line which expressing NME4 gene fused with SFB triple tags (S tag-2x Flag tag-SBP tag) or TurboID. We isolated, comb...
ORGANISM(S): Homo sapiens (Human) 
2023-10-13 | PXD036624 | Pride
Tap-ms turboid-ms 
ProteomicsMapping the protein interaction network of adaptor protein complex in Arabidopsis identifies P34 as a common stability regulator
Adaptor protein (AP) complexes are evolutionarily conserved vesicle transport regulators that recruit coat proteins, membrane cargos and coated vesicle accessory proteins. Since in plants endocytic and post-Golgi trafficking intersect at the trans-Golgi network, unique mechanisms for sorting cargos ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2023-12-07 | PXD035454 | Pride
Ap-ms Arabidopsis Turboid Proximity labeling Adaptor complex Tap Interaction network 
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