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TranscriptomicsIdentification of glucose-TOR signaling early target genes in Arabidopsis seedling autotrophic transition stage
The goal of this experiment was to explore the molecular network of glucose-TOR signaling in Arabidopsis seedling autotrophic transition stage. We used the whole-genome microarrays to detail the global program of gene expression mediated by glucose and TOR. Arabidopsis WT and estradiol inducible RNA...
ORGANISM(S): Arabidopsis thaliana 
2013-04-05 | E-GEOD-40245 | biostudies-arrayexpress
TranscriptomicsDifferentially regulated genes in TOR knockdown Arabidopsis lines
Rapamycin-sensitive transgenic Arabidopsis lines (BP12) expressing yeast FK506 Binding Protein12 (FKBP12) were developed. Inhibition of TOR in BP12 plants by rapamycin resulted in slower overall root, leaf and shoot growth and development leading to poor nutrient uptake and light energy utilization....
ORGANISM(S): Arabidopsis thaliana 
2012-12-18 | E-GEOD-42968 | biostudies-arrayexpress
TranscriptomicsIsp7 is a novel regulator of amino acid uptake in the TOR signaling pathway
We show that the sensitivity of tsc mutant cells to rapamycin is mediated by TORC1 and can be suppressed by overexpression of the 2-oxoglutarate-Fe(II) dependent oxygenase, Isp7. We show that Isp7 is a novel regulator of amino acids uptake that acts via regulation of gene expression, both upstream a...
ORGANISM(S): Schizosaccharomyces pombe 
2014-02-05 | E-GEOD-52759 | biostudies-arrayexpress
TranscriptomicsSch9 Is a Major Target of TORC1 in Saccharomyces cerevisiae
The Target Of Rapamycin (TOR) protein is a Ser/Thr kinase that functions in two distinct multiprotein complexes: TORC1 and TORC2. These conserved complexes regulate many different aspects of cell growth in response to intra- and extracellular cues. Here we report the first bona fide substrate of yea...
ORGANISM(S): Saccharomyces cerevisiae 
2010-09-15 | E-GEOD-7660 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of yeast gcn2, gcn4 and gln3 deletion mutants treated with rapamycin or 3-amino-1,2,4-triazole (3-AT)
Two nutrient sensing and regulatory pathways, the general amino acid control (GAAC) and the target of rapamycin (TOR), control yeast growth and metabolism in response to changes in nutrient availability. Starvation for amino acids activates the GAAC pathway, involving Gcn2p phosphorylation of eIF2 ...
ORGANISM(S): Saccharomyces cerevisiae 
2010-03-07 | E-GEOD-15254 | biostudies-arrayexpress
TranscriptomicsIdentification of new target genes of TOR during growth in MDN
To identify novel targets of TOR when prototrophic strain S288c is grown in MDN media. Arrays compare growth in MDN with and without addition of rapamycin. For comparison, growth in YPD versus MDN as well as SCD versus MDN were examined as well.
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-30 | E-GEOD-3157 | biostudies-arrayexpress
TranscriptomicsSynergistic effects of TOR and proteasome pathways on the yeast transcriptome and cell growth
The experiments were designed to interrogate the genetic interaction relationship between the TOR signalling pathway and the UPS-proteasome pathway in gene expression and cell growth. Yeast cells (BY4742pdr5delta) were treated with the drug vehicle, MG132 (50uM), rapamycin (200ng/ml) or both drugs. ...
ORGANISM(S): Saccharomyces cerevisiae 
2013-05-24 | E-MTAB-1550 | biostudies-arrayexpress
ProteomicsFunctional Characterization of Target of Rapamycin (TOR) Signalling in Physcomitrella
Target of rapamycin (TOR) is a structurally and functionally conserved protein kinase and an important signalling hub in eukaryotes. The moss Physcomitrella (Physcomitrium patens) is a model organism for plant physiology, development, and evolution. However, little is known about TOR signalling in n...
ORGANISM(S): Physcomitrella Physcomitrella patens subsp. patens (Moss) 
2026-02-09 | PXD069889 | Pride
Physcomitrella Tor signalling 
TranscriptomicsRapamycin effect on gene expression: dependence on c-Myc
This experiment utilized rat fibroblasts that are wild-type for c-Myc (TGR-1 cells) and null for c-Myc (HO15.19 cells). Both were treated treated with rapamycin for 24 hr (comparison group, vehicle control). Cells were studied under conditions that supported proliferation. Rapamycin (50nM or DMSO ...
ORGANISM(S): Rattus norvegicus 
2010-10-25 | E-GEOD-18845 | biostudies-arrayexpress
TranscriptomicsTranscriptional profiling of Rapamycin treatment in yeast cells lacking the Ptc1 protein phosphatase.
We are interested in the study of the already reported sensitivity of yeast cells lacking the type 1 protein phosphatase Ptc1 to the drug rapamycin. In order to carry out our studies, we analyzed the changes in the transcription profile that a short-term incubation with rapamycin (200 ng/mL) has in ...
ORGANISM(S): Saccharomyces cerevisiae 
2008-12-31 | E-GEOD-11530 | biostudies-arrayexpress
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