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ProteomicsQuantitative thiol reactivity profiling
Cysteine is unique among all protein-coding amino acids owing to its intrinsically high nucleophilicity. The cysteinyl thiol group can be covalently modified by both a broad range of redox mechanisms and various electrophiles derived from exogenous or endogenous sources. Measuring proteomic cysteine...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2020-07-24 | PXD016048 | Pride
Thiol Redox Chemical proteomics Electrophile 
ProteomicsMultiplexed thiol reactivity profiling for target discovery of electrophilic natural products
Electrophilic groups, such as Michael acceptors, expoxides, are common motifs in natural products (NPs). Electrophilic NPs can act through covalent modification of cysteinyl thiols on functional proteins, and exhibit potent cytotoxicity and anti-inflammatory/cancer activities. Here we describe a new...
ORGANISM(S): Homo sapiens (Human) 
2017-10-12 | PXD007575 | Pride
Mtrp natural products chemoproteomics 
ProteomicsDevelopment of a maleimide-based chemical proteomic method for quantitative analysis of cysteine reactivity
Cysteine is the most intrinsically nucleophilic residue in proteins and serves as a sentinel against increasing reactive oxygen species (ROS) via reversible thiol oxidation. Despite the importance of Cys oxidation in understanding biological stress response, it remains largely unknown and a major an...
ORGANISM(S): Chlamydomonas reinhardtii 
2020-10-20 | PXD015409 | Pride
Thiol-reactivity profiling Chlamydomonas reinhardtii Chemical proteomics Cysteine oxidation 
UnknownScalable Thiol Reactivity Profiling Identifies Azetidinyl Oxadiazoles as Cysteine-Targeting Electrophiles.
Not available
S-EPMC11995717 | biostudies-literature
ProteomicsAn Anti-Cancer Gold(III)-Activated Porphyrin Scaffold that Covalently Modifies Protein Cysteine Thiols
Cysteine thiols of many cancer-associated proteins are attractive targets of anti-cancer agents. Herein, we unequivocally demonstrate a distinct thiol-targeting property of gold(III) mesoporphyrin IX dimethyl ester (AuMesoIX) and its anti-cancer activities. While the binding of cysteine thiols with ...
ORGANISM(S): Homo sapiens (Human) 
2021-09-08 | PXD016875 | Pride
Gold • porphyrin • ligand reactivity• cysteine thiol conjugation • anti-tumor agents 
UnknownEvaluating the utility of a high throughput thiol-containing fluorescent probe to screen for reactivity: A case study with the Tox21 library.
Not available
S-EPMC10304587 | biostudies-literature
TranscriptomicsQuantitative mRNA expression comparison of Hepatitis C Virus replicon (2a) on Huh7.5 cell lines
We developed transcriptome expression assisted non-directed proteome profiling (TEAnDPP) method to investigate host-pathogen interaction. Analysis of HCV replicon induced host-cell metabolism perturbation at gene expression level. Gene enrichment analysis on DEG revealed disulfide formation related ...
ORGANISM(S): Homo sapiens 
2014-10-21 | GSE62546 | GEO
ProteomicspChem_benchmarking_dataset_QTRP_diff_instruments
Data sets are used for benchmarking, pChem, a modification-centric, blind-search tool to provide a streamlined pipeline for unbiased assessing of the performance of chemoproteomic probes. The IPM-based QTRP (quantitative thiol reactivity profiling) data sets were recorded on different LC-MS/MS instr...
ORGANISM(S): Homo sapiens (Human) 
2022-12-16 | PXD027758 | Pride
Instruments pchem 
UnknownIsotopically-Labeled Iodoacetamide-Alkyne Probes for Quantitative Cysteine-Reactivity Profiling.
Not available
S-EPMC5841085 | biostudies-literature
ProteomicspChem_benchmarking_dataset_QTRP_diff_species
Data sets are used for benchmarking, pChem, a modification-centric, blind-search tool to provide a streamlined pipeline for unbiased assessing of the performance of chemoproteomic probes. Data were produced from the IPM-based QTRP (quantitative thiol reactivity profiling) applications in various spe...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Mus musculus (Mouse) Homo sapiens (Human) Escherichia coli Drosophila melanogaster (Fruit fly) Caenorhabditis elegans Rattus norvegicus (Rat) 
2024-09-24 | PXD027767 | Pride
Ipm diff_species pchem 
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