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ProteomicsMurine kidney glomeruli phosphorylation
Isolated murine kidney glomeruli were lysed in buffer containing 8M urea, and proteins were reduced and alkylated. Proteins were digested with trypsin. After reverse-phase chomatrography, peptides were fractionated using strong cation exchange chromatography. Phosphopeptides were enriched using IMAC...
ORGANISM(S): Mus musculus (Mouse) 
2016-05-09 | PXD000266 | Pride
Mouse Podocytes Kidney Glomerular epithelial cell Phosphoproteomics 
TranscriptomicsMonocyte to macrophage differentiation and loading/deloading with lipoproteins (eLDL, oxLDL, HDL3)
Elutriated monocytes from 9 healthy donors with ApoE3/E3 genotype were differentiated for up to six days in vitro to macrophages using rhMCSF under serum-free conditions. Samples were taken on days 1, 4, 5 and 6 and analyzed for their transcriptomic profiles. After four days of differentiation cells...
ORGANISM(S): Homo sapiens 
2014-03-02 | E-MTAB-2298 | biostudies-arrayexpress
ModelsMiddleton2012_GibberellinSignalling
This model is from the article: Mathematical modeling elucidates the role of transcriptional feedback in gibberellin signaling. Middleton AM , Úbeda-Tomás S , Griffiths J , Holman T , Hedden P , Thomas SG , Phillips AL , Holdsworth MJ , Bennett MJ , K...
2024-09-02 | BIOMD0000000422 | BioModels
ProteomicsAnalysis of N-Glycosylation for SARS-CoV-2 expressed receptor-binding domain VOCs and membrane protein with LC-MS.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can infect human cells by first attaching to the ACE-2 receptor via its receptor-binding domain (RBD) in the spike protein. We investigated the influence of N-glycosylation sites of the RBD and the membrane (M) protein on IgG antibody bind...
ORGANISM(S): Severe Acute Respiratory Syndrome Coronavirus 2 
2024-04-29 | PXD048930 | panorama
TranscriptomicsIdentification of mRNAs bound and regulated by human LIN28 proteins and molecular requirements for RNA recognition [PAR-CLIP]
Human LIN28A and B are RNA-binding proteins (RBPs) conserved in animals with important roles during development and stem cell reprogramming. We used Photoactivatable-Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation (PAR-CLIP) in HEK293 cells and identified a largely overlapping set of ~3...
ORGANISM(S): Homo sapiens 
2013-02-26 | E-GEOD-44615 | biostudies-arrayexpress
MetabolomicsFirst-in-class MKK4 inhibitors enhance liver regeneration and prevent liver failure

Diminished hepatocyte regeneration is a key feature of acute and chronic liver diseases and after extended liver resections, resulting in the inability to maintain or restore a sufficient functional liver mass. Therapies to restore hepatocyte regeneration are lacking, making liver transplantation...

2024-09-23 | MTBLS9434 | MetaboLights
TranscriptomicsIdentification of mRNAs bound and regulated by human LIN28 proteins and molecular requirements for RNA recognition [RNA-Seq]
Human LIN28A and B are RNA-binding proteins (RBPs) conserved in animals with important roles during development and stem cell reprogramming. We used Photoactivatable-Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation (PAR-CLIP) in HEK293 cells and identified a largely overlapping set of ~3...
ORGANISM(S): Homo sapiens 
2013-02-26 | E-GEOD-44568 | biostudies-arrayexpress
TranscriptomicsIdentification of mRNAs bound and regulated by human LIN28 proteins and molecular requirements for RNA recognition [Affymetrix]
Human LIN28A and B are RNA-binding proteins (RBPs) conserved in animals with important roles during development and stem cell reprogramming. We used Photoactivatable-Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation (PAR-CLIP) in HEK293 cells and identified a largely overlapping set of ~3...
ORGANISM(S): Homo sapiens 
2013-02-26 | E-GEOD-44613 | biostudies-arrayexpress
TranscriptomicsGene expression profiling of 6 acute hepatitis C patients
Approximately 50% of patients with chronic hepatitis C (CHC) have a sustained virologic response (SVR) to treatment with pegylated interferon (pegINF)-M-NM-1 and ribavirin. Non-response to treatment is associated with constitutively increased expression of IFN-stimulated genes (ISGs) in the liver. T...
ORGANISM(S): Homo sapiens 
2012-06-08 | E-GEOD-38597 | biostudies-arrayexpress
TranscriptomicsCharacterization of AGO1-/AGO4-associated smRNAs
To fully characterize smRNAs associated with AGO1 and AGO4, we developed a two-step protocol to purify AGO/smRNA complexes from flowers, leaves, roots and seedlings with enhanced purity, and sequenced the smRNAs by IlluminaM-CM-"M-BM-^@M-BM-^Ys technology. We identified some additional miRNAs, colla...
ORGANISM(S): Arabidopsis thaliana 
2011-04-19 | E-GEOD-28591 | biostudies-arrayexpress
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